[培养的CD45/CD31小鼠肺侧群细胞向血管平滑肌细胞的分化]
[Differentiation of Cultivated CD45/CD31 Mouse Lung Side Population Cells into Vascular Smooth Muscle Cells ].
作者信息
Zhang Ming-Jie, Li Zong-Ze, Xu Yang, Liang Simon Xun
机构信息
Department of Biochemistry and Molecular Biology , Jinzhou Medical University, Jinzhou 121000, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2017 May;48(3):363-367.
OBJECTIVES
To investigate the characteristics of differentiation of lung side population cells (LSP cells).
METHODS
CD45/CD31 LSP cells sorted by flow cytometry were taken from mouse lung tissues and cultured for 14 d. The cultured LSP cells were observed with colony formation assay and flow cytometry. The mRNA expressions of ATP-binding cassette transporter G2 (), smooth muscle actin () and α-smooth muscle tropomyosin () in both freshly isolated LSP cells and cultured LSP cells were examined. The expressions of ABCG2 and stem cell antigen 1 (Sca1) in LSP cells were detected using immunofluorescence. RT-PCR tests were performed to detect the expressions of ABCG2, SMA and α-SMT in LSP cells.
RESULTS
The isolated CD45/CD31 lung side population cells expressed ABCG2, and Sca1, but not . A large number of LSP in aggregated state were observed after 14 d of culture. Before induction of differentiation, the CD45/CD31 LSP cells expressed and , but not α-SMT. After induction of differentiation, the CD45/CD31 lung side population cells expressed α-SMT and , but not .
CONCLUSIONS
CD45/CD31 LSP cells might be progenitor cells of vascular smooth muscle cells, possessing the characteristics of stem cell differentiations.
目的
研究肺侧群细胞（LSP细胞）的分化特性。
方法
通过流式细胞术分选的CD45/CD31 LSP细胞取自小鼠肺组织，并培养14天。用集落形成试验和流式细胞术观察培养的LSP细胞。检测新鲜分离的LSP细胞和培养的LSP细胞中ATP结合盒转运体G2（ABCG2）、平滑肌肌动蛋白（SMA）和α-平滑肌原肌球蛋白（α-SMT）的mRNA表达。用免疫荧光法检测LSP细胞中ABCG2和干细胞抗原1（Sca1）的表达。进行RT-PCR试验检测LSP细胞中ABCG2、SMA和α-SMT的表达。
结果
分离的CD45/CD31肺侧群细胞表达ABCG2、Sca1，但不表达α-SMT。培养14天后观察到大量聚集状态的LSP。在诱导分化前，CD45/CD31 LSP细胞表达SMA，但不表达α-SMT。诱导分化后，CD45/CD31肺侧群细胞表达α-SMT，但不表达SMA。
结论
CD45/CD31 LSP细胞可能是血管平滑肌细胞的祖细胞，具有干细胞分化的特性。
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