用于蛋白质结构与功能研究的新型核磁共振工具：用于动态核极化固态核磁共振的自旋标记
New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.
作者信息
Rogawski Rivkah, McDermott Ann E
机构信息
Department of Chemistry, Columbia University, NY, NY 10027, United States.
Department of Chemistry, Columbia University, NY, NY 10027, United States.
出版信息
Arch Biochem Biophys. 2017 Aug 15;628:102-113. doi: 10.1016/j.abb.2017.06.010. Epub 2017 Jun 13.
Abstract
Magic angle spinning solid state NMR studies of biological macromolecules [1-3] have enabled exciting studies of membrane proteins [4,5], amyloid fibrils [6], viruses, and large macromolecular assemblies [7]. Dynamic nuclear polarization (DNP) provides a means to enhance detection sensitivity for NMR, particularly for solid state NMR, with many recent biological applications and considerable contemporary efforts towards elaboration and optimization of the DNP experiment. This review explores precedents and innovations in biological DNP experiments, especially highlighting novel chemical biology approaches to introduce the radicals that serve as a source of polarization in DNP experiments.
摘要
生物大分子的魔角旋转固态核磁共振研究[1 - 3]推动了对膜蛋白[4,5]、淀粉样原纤维[6]、病毒以及大型大分子聚集体[7]的激动人心的研究。动态核极化（DNP）提供了一种增强核磁共振检测灵敏度的方法，特别是对于固态核磁共振，近期有许多生物学应用，并且当前为详细阐述和优化DNP实验付出了相当大的努力。本综述探讨了生物DNP实验中的先例和创新，尤其着重介绍了引入在DNP实验中作为极化源的自由基的新型化学生物学方法。
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