Phinney Karen W, Camara Johanna E, Tai Susan S-C, Sander Lane C, Wise Stephen A, De Grande Linde A C, Thienpont Linda M, Possolo Antonio M, Toman Blaza, Sempos Christopher T, Betz Joseph M, Coates Paul M
National Institute of Standards and Technology, Biomolecular Measurement Division and Chemical Sciences Division, Gaithersburg, MD 20899.
Ghent University, Faculty of Pharmaceutical Sciences, Laboratory for Analytical Chemistry, Ghent, Belgium.
J AOAC Int. 2017 Sep 1;100(5):1253-1259. doi: 10.5740/jaoacint.17-0204. Epub 2017 Jun 16.
Assay variability has been cited as an obstacle to establishing optimal vitamin D exposure. As part of the Vitamin D Standardization Program (VDSP) effort to standardize the measurement of total 25-hydroxyvitamin D [25(OH)D], the value assignment of total 25(OH)D in 50 single-donor serum samples was performed using two isotope-dilution LC with tandem MS methods. Both methods are recognized as reference measurement procedures (RMPs) by the Joint Committee for Traceability in Laboratory Medicine. These samples and their assigned values serve as the foundation for several aspects of the VDSP. To our knowledge, this is the first time that two RMPs have been used to assign 25(OH)D values to such a large number of serum samples.
检测变异性被认为是确定最佳维生素D暴露量的一个障碍。作为维生素D标准化计划（VDSP）使总25-羟基维生素D [25(OH)D] 测量标准化工作的一部分，采用两种同位素稀释液相色谱串联质谱法对50份单供体血清样本中的总25(OH)D进行了赋值。这两种方法均被实验室医学溯源联合委员会认可为参考测量程序（RMP）。这些样本及其赋值是VDSP多个方面的基础。据我们所知，这是首次使用两种RMP对如此大量的血清样本进行25(OH)D值的赋值。
