Wolfson Nanomaterials and Devices Laboratory, School of Computing, Electronics and Mathematics, Faculty of Science and Engineering, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Biosensors (Basel). 2017 Jul 12;7(3):27. doi: 10.3390/bios7030027.
We report on a label-free immunosensor based on graphene field effect transistors (G-FETs) for the ultrasensitive detection of Human Chorionic Gonadotrophin (hCG), as an indicator of pregnancy and related disorders, such as actopic pregnancy, choriocarcinoma and orchic teratoma. Pyrene based bioactive ester was non-covalently anchored onto the graphene channel in order to retain the sp² lattice. The G-FET transfer characteristics showed repeatable and reliable responses in all surface modifying steps using a direct current (DC) readout system. The hCG concentration gradient showed a detection limit of ~1 pg·mL. The proposed method facilitates the cost-effective and viable production of graphene point-of-care devices for clinical diagnosis.
我们报告了一种基于石墨烯场效应晶体管（G-FET）的无标记免疫传感器，用于超灵敏检测人绒毛膜促性腺激素（hCG），hCG 是人妊娠和相关疾病（如宫外孕、绒毛膜癌和卵巢畸胎瘤）的标志物。基于芘的生物活性酯通过非共价键锚定在石墨烯通道上，以保留 sp² 晶格。使用直流（DC）读出系统，G-FET 传输特性在所有表面修饰步骤中均表现出可重复且可靠的响应。hCG 浓度梯度的检测限约为 1 pg·mL。该方法有利于基于石墨烯的即时诊断设备的经济高效和可行生产。
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