高动态范围荧光层流光学断层扫描（HDR-FLOT）。
High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT).
作者信息
Tang Qinggong, Liu Yi, Tsytsarev Vassiliy, Lin Jonathan, Wang Bohan, Kanniyappan Udayakumar, Li Zhifang, Chen Yu
机构信息
Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
Contributed equally.
出版信息
Biomed Opt Express. 2017 Mar 9;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 1.
Abstract
Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging
摘要
三维荧光层状光学断层扫描（FLOT）能够实现100-200微米的分辨率以及2-3毫米的穿透深度。FLOT已应用于组织工程、神经科学以及肿瘤学领域。基于电荷耦合器件的系统动态范围有限，使得难以对具有大浓度差异的荧光样本进行成像，限制了其穿透深度，并降低了三维重建数据的定量准确性。在此，我们引入了数码相机中广泛使用的高动态范围（HDR）方法，提出了HDR-FLOT，增加了穿透深度并提高了对具有大浓度差异的荧光样本进行成像的能力。该方法使用琼脂模型和B6小鼠进行脑成像测试。
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