Bakir Hanaa Y, Ah Attia Rasha, Mahmoud Abeer E, Ibraheim Zedan
Dept. of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Dept. of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt.
Iran J Parasitol. 2017 Apr-Jun;12(2):188-195.
The protective response developed against infection provokes immune and inflammatory responses mediated by cytokines released from T helper cells. We aimed to evaluate the effect of albendazole or myrrh on the expression of IFN-γ and IL-10 in BALB/c mice infected with .
This study was done at the Animal House of Faculty of Medicine, Assiut University (Assiut, Egypt) from April to December 2015. Mice were infected with 300 larvae and treated with albendazole (50 mg/kg per day) or myrrh (500 mg/kg per day) for 3 consecutive days post-infection (pi). The expression of INF-γ and IL-10 was detected in the intestinal tissue by reverse transcription (RT) PCR.
The expression of IFN-γ in mice treated with albendazole and myrrh was detected on days 3 and 15 pi respectively. In the control group, it was found on days 5, 10, 15 and 20 pi with the highest expression on day 15 pi. The expression of IL-10 was detected on days 3, 20 pi in the albendazole and myrrh treated groups, respectively. In the control group, IL-10 expression appeared on days 5 and day 20 pi.
The target of albendazole and myrrh on the profile of IFN-γ and IL-10 on these cytokines were encouraging to reinforce their therapeutic use against trichinellosis.
针对感染产生的保护性反应会引发由辅助性T细胞释放的细胞因子介导的免疫和炎症反应。我们旨在评估阿苯达唑或没药对感染[具体病原体未提及]的BALB/c小鼠中IFN-γ和IL-10表达的影响。
本研究于2015年4月至12月在艾斯尤特大学医学院动物房（埃及艾斯尤特）进行。小鼠感染300条[具体幼虫未提及]幼虫，并在感染后连续3天用阿苯达唑（每天50 mg/kg）或没药（每天500 mg/kg）进行治疗。通过逆转录（RT）PCR检测肠道组织中INF-γ和IL-10的表达。
用阿苯达唑和没药治疗的小鼠中，IFN-γ的表达分别在感染后第3天和第15天被检测到。在对照组中，在感染后第5天、第10天、第15天和第20天检测到，第15天表达最高。IL-10的表达分别在阿苯达唑和没药治疗组的感染后第3天和第20天被检测到。在对照组中，IL-10表达出现在感染后第5天和第20天。
阿苯达唑和没药对IFN-γ和IL-10细胞因子谱的作用令人鼓舞，可加强它们在旋毛虫病治疗中的应用。
