伊朗南部特定人群中临床分离株存在相当大的遗传多样性。
Considerable Genetic Diversity of Clinical Isolates in a Targeted Population in South of Iran.
作者信息
Tavakoli Oliaee Razieh, Babaei Zahra, Hatam Gholam Reza, Tavakoli Kareshk Amir, Mahmoudvand Hosein, Vafafar Arghavan, Ziaali Naser
机构信息
Dept. of Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
Iran J Parasitol. 2017 Apr-Jun;12(2):251-259.
BACKGROUND
The present study aimed to characterize genetically and to compare the most frequently occurring strains of isolated from southern Iran.
METHODS
Totally, 150 vaginal swab and urine specimens were collected from symptomatic and asymptomatic women from May 2012 to Jun 2013. This study implemented a sensitive and reliable PCR-restriction fragment length polymorphism (RFLP) typing method on the actin gene. Moreover, one representative sample of each identified genotype was subjected to sequencing.
RESULTS
Twenty-four isolates were positive and 6 distinct electrophoretic patterns (H, E, G, I, M, N) were identified. Genotypes H and I were found to be more prevalent (50 and 37.5%) in Kerman and Shiraz, respectively. The phylogenetic analysis showed that two isolates were located as a separated clade with the other isolates.
CONCLUSION
The obtained findings showed a considerable genetic polymorphism of clinical isolates from the population studied. More studies may be warranted in future as to unveiling any possible links between a given genotype/cluster and pathogenic behavior of
背景
本研究旨在对从伊朗南部分离出的最常见菌株进行基因特征分析并比较。
方法
2012年5月至2013年6月期间，共从有症状和无症状女性中收集了150份阴道拭子和尿液标本。本研究对肌动蛋白基因采用了一种灵敏且可靠的聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分型方法。此外，对每种鉴定出的基因型的一个代表性样本进行测序。
结果
24株分离株呈阳性，鉴定出6种不同的电泳图谱（H、E、G、I、M、N）。发现基因型H和I在克尔曼和设拉子分别更为普遍（分别为50%和37.5%）。系统发育分析表明，有两株分离株与其他分离株位于一个单独的分支中。
结论
所获结果表明，在所研究人群的临床分离株中存在相当大的基因多态性。未来可能需要进行更多研究，以揭示特定基因型/聚类与……致病行为之间的任何可能联系。
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