Defined Combinations of Cryomedia and Thawing Extenders Influence the Viable X-Y Boar Sperm Ratio in Vitro.
作者信息
Korchunjit W, Kaeoket K, Kitiyanant Y, Taylor J, Wongtawan T
机构信息
Laboratory of Cellular Biomedicine and Veterinary Medicine, Faculty of Veterinary Science, Mahidol University, Salaya campus, Phuttamonthon, Nakhon Pathom, Thailand.
Semen Laboratory; Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, Thailand.
出版信息
Cryo Letters. 2017 May/Jun;38(3):160-165.
BACKGROUND
It is believed that plasma membrane X- and Y-chromosome bearing sperm are different; therefore the freezing and thawing process may affect X- and Y-sperm differently.
OBJECTIVE
The objective of this study was to investigate the effect of cryomedia and thawing extenders on the survival of X and Y-sperm.
MATERIALS AND METHODS
Three different cryomedia and thawing extenders were compared. Viable motile sperm were separated using a swim-up technique. Real-time PCR was used to identify the sperm type.
RESULTS
Using CryoA for freezing and Beltsville-Thawing-Solution (BTS) as the thawing extender yielded significantly higher numbers of viable motile Y sperm (64 percent) than control (48 percent) (P < 0.01). Conversely, semen freezing with CryoC and thawing with Androstar Plus gave a significantly lower number of viable motile Y sperm (32 percent) than control (51 percent).
CONCLUSION
Our results revealed that defined combinations of cryomedia and thawing extenders significantly altered the survival ratio of frozen-thawed X-Y sperm in vitro, which has potential implications for artificial insemination.
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