Fu Li, Wang Zhanfeng, Dong Shujun, Cai Yan, Ni Yuxin, Zhang Tianshou, Wang Lin, Zhou Yanmin
Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.
Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun 130033, China.
Materials (Basel). 2017 Mar 3;10(3):257. doi: 10.3390/ma10030257.
Guided bone regeneration (GBR) is one such treatment that reconstructs neo-bone tissue by using a barrier membrane to prevent the invasion of soft tissue and to create a space for guiding new bone growth into the bone defect. Herein, we report a novel functionally graded bilayer membrane (FGBM) for GBR application. To fabricate the novel membrane, the composites of poly(lactic-co-glycolic acid) and nano-hydroxyapatite were prepared by phase inversion for the dense layer and by electrospinning for another porous layer, and their corresponding properties were evaluated including surface morphology, mechanics, degradability, cell barrier function, and in vitro osteogenic bioactivity. The results showed that PLGA with 5% nHA in dense layer could meet the requirement of mechanical strength and have excellent barrier function even on condition of post-degradation. Furthermore, PLGA with 30% nHA in porous layer could achieve the good physical and chemical properties. In addition, 30% nHA incorporation would enhance the in vitro mineralization, and have superior capabilities of cell adhesion, proliferation and differentiation compared to other groups. Therefore, the designed FGBM could potentially serve as a barrier for preferential tissue ingrowth and achieve a desirable therapeutic result for bone tissue regeneration.
引导骨再生（GBR）就是这样一种治疗方法，它通过使用屏障膜来重建新骨组织，以防止软组织侵入并为引导新骨生长到骨缺损部位创造空间。在此，我们报道一种用于GBR应用的新型功能梯度双层膜（FGBM）。为制备这种新型膜，聚乳酸 - 乙醇酸共聚物（PLGA）与纳米羟基磷灰石（nHA）的复合材料通过相转化法制备致密层，通过静电纺丝法制备另一多孔层，并对其相应性能进行了评估，包括表面形态、力学性能、降解性、细胞屏障功能和体外成骨生物活性。结果表明，致密层中含有5% nHA的PLGA能够满足机械强度要求，即使在降解后仍具有优异的屏障功能。此外，多孔层中含有30% nHA的PLGA能够实现良好的物理和化学性能。另外，掺入30% nHA会增强体外矿化作用，并且与其他组相比具有更强的细胞黏附、增殖和分化能力。因此，所设计的FGBM有可能作为优先组织向内生长的屏障，并为骨组织再生实现理想的治疗效果。
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