血糖估计方法：比较评估
METHODS FOR ESTIMATION OF BLOOD GLUCOSE : A COMPARATIVE EVALUATION.
作者信息
Ambade Vivek N, Sharma Y V, Somani B L
机构信息
Scientist C, Armed Forces Medical College, Pune 411040.
Professor and Head, Armed Forces Medical College, Pune 411040.
出版信息
Med J Armed Forces India. 1998 Apr;54(2):131-133. doi: 10.1016/S0377-1237(17)30502-6. Epub 2017 Jun 26.
Glucose in blood is the most frequent analyzed parameter in a clinical chemistry laboratory. In Armed Forces Laboratories, copper reduction method (Modified Folin Wu) is commonly used. Here we have compared this method as well as O-Toluidine and GOD-POD method with reference UV-Hexokinase method. Both Modified Folin Wu and O-Toluidine showed upward deviation with substantial imprecision (CV=4.9% - 3.5% and 6% - 5.8% respectively) and inaccuracy (Average deviation = 5.76% and 10.68% respectively). GOD-POD method was found linear (up to 500 mg/dl), with good precision (CV=0.7% to 1.4%) and accuracy (Average deviation= -0.97%). This method is simple, rapid, economical and sensitive, and can be adopted to a routine colorimeter.
血液中的葡萄糖是临床化学实验室中最常分析的参数。在军队实验室中，常用铜还原法（改良福林-吴法）。在此，我们将该方法以及邻甲苯胺法和葡萄糖氧化酶-过氧化物酶法与参考紫外-己糖激酶法进行了比较。改良福林-吴法和邻甲苯胺法均显示出向上偏差，且精密度较差（变异系数分别为4.9% - 3.5%和6% - 5.8%）以及准确性较差（平均偏差分别为5.76%和10.68%）。葡萄糖氧化酶-过氧化物酶法呈线性（高达500mg/dl），具有良好的精密度（变异系数=0.7%至1.4%）和准确性（平均偏差=-0.97%）。该方法简单、快速、经济且灵敏，可用于常规比色计。
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