Kooshki Razieh, Abbasnejad Mehdi, Esmaeili-Mahani Saeed, Raoof Maryam
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.
Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Basic Clin Neurosci. 2017 May-Jun;8(3):213-222. doi: 10.18869/nirp.bcn.8.3.213.
Cognitive impairment is commonly associated with pain. The modulatory role of (OX1R) in pain pathways as well as learning and memory processes is reported in several studies. The current study was designed to investigate the possible role of CA1-hippocampal OX1R on spatial learning and memory of rats following capsaicin-induced orofacial pain.
Orofacial pain was induced by subcutaneous intra lip injection of capsaicin (100 μg). CA1 administration of orexin A and its selective antagonist (SB-334867-A) were performed 20 minutes prior to capsaicin injection. Learning and spatial memory performances were assessed by Morris Water Maze (MWM) task.
Capsaicin treated rats showed impairment in spatial learning and memory. In addition, pretreatment with orexin A (20 and 40 nM/rat) significantly attenuated learning and memory impairment in capsaicin-treated rats. Conversely, blockage of OX1R via SB-334867-A (40 and 80 nM/rat) significantly exaggerated learning and memory loss in capsaicin-treated rats.
The obtained results indicated that CA1 OX1R may be involved in modulation of capsaicin -induced spatial learning and memory impairment.
认知障碍通常与疼痛相关。多项研究报道了（OX1R）在疼痛通路以及学习和记忆过程中的调节作用。本研究旨在探讨CA1海马区OX1R在辣椒素诱导的口面部疼痛后对大鼠空间学习和记忆的可能作用。
通过皮下唇部注射辣椒素（100μg）诱导口面部疼痛。在注射辣椒素前20分钟，对CA1区给予食欲素A及其选择性拮抗剂（SB-334867-A）。通过莫里斯水迷宫（MWM）任务评估学习和空间记忆表现。
辣椒素处理的大鼠在空间学习和记忆方面表现出损伤。此外，用食欲素A（20和40 nM/大鼠）预处理可显著减轻辣椒素处理大鼠的学习和记忆损伤。相反，通过SB-334867-A（40和80 nM/大鼠）阻断OX1R可显著加剧辣椒素处理大鼠的学习和记忆丧失。
所得结果表明，CA1区OX1R可能参与调节辣椒素诱导的空间学习和记忆损伤。
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