不同牙种植体-基台组合体释放的金属离子对成骨细胞功能和骨吸收介质分泌的影响。

The effect of metal ions released from different dental implant-abutment couples on osteoblast function and secretion of bone resorbing mediators.

机构信息

Prosthodontic Unit, Department of Restorative Dentistry, UCL Eastman Dental Institute, University College London, London, UK; Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia,; Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK.

Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK.

出版信息

J Dent. 2017 Nov;66:91-101. doi: 10.1016/j.jdent.2017.08.002. Epub 2017 Aug 4.

DOI:10.1016/j.jdent.2017.08.002

PMID:28800964

Abstract

OBJECTIVES

The etiology of the reduced marginal bone loss observed around platform-switched implant-abutment connections is not clear but could be related to the release of variable amounts of corrosion products. The present study evaluated the effect of different concentrations of metal ions released from different implant abutment couples on osteoblastic cell viability, apoptosis and expression of genes related to bone resorption.

METHODS

Osteoblastic cells were exposed to five conditions of culture media prepared containing metal ions (titanium, aluminum, vanadium, cobalt, chromium and molybdenum) in different concentrations representing the amounts released from platform-matched and platform-switched implant-abutment couples as a result of an earlier accelerated corrosion experiment. Cell viability was evaluated over 21days using the Alamar Blue assay. Induction of apoptosis was measured after 24h of exposure using flow cytometry. Expression of interleukin-6, interleukin-8, cyclooxygenase-2, caspase-8, osteoprotegerin and receptor activator of nuclear factor kappa-B ligand (RANKL) by osteoblastic cells were analysed after exposure for 1, 3 and 21days using real-time quantitative polymerase chain reaction assay RESULTS: Metal ions in concentrations representing the platform-matched groups led to a reduction in cell viability (P<0.01) up to 7days of exposure. Stimulated cells showed higher rates of early apoptosis (P<0.01) compared to non-treated cells. Metal ions up-regulated the expression of interleukin-6, interleukin-8, cyclooxygenase-2 and RANKL in a dose dependent manner after 1day of exposure (P<0.05). The up-regulation was more pronounced in the groups containing the corrosion products of platform-matched implant-abutment couples.

CONCLUSION

Osteoblastic cell viability, apoptosis, and regulation of bone resorbing mediators were significantly altered in the presence of metal ions. The change in cytokine levels expressed was directly proportional to the metal ion concentration.

CLINICAL SIGNIFICANCE

The observed biological responses to decreased amounts of metal ions released from platform-switched implant-abutment couples compared to platform-matched couples may partly explain the positive radiographic findings in respect to crestal bone level when utilising the "platform-switching" concept, which highlights the possible role of corrosion products in the mediation of crestal bone loss around dental implants.

摘要

目的

平台转换种植体-基台连接周围观察到的边缘骨吸收减少的病因尚不清楚，但可能与释放不同量的腐蚀产物有关。本研究评估了来自不同种植体-基台组合的不同浓度金属离子对成骨细胞活力、细胞凋亡和与骨吸收相关基因表达的影响。

方法

将成骨细胞暴露于含有金属离子（钛、铝、钒、钴、铬和钼）的五种培养条件下，浓度代表早期加速腐蚀实验中从平台匹配和平台转换种植体-基台组合中释放的量。使用 Alamar Blue 测定法在 21 天内评估细胞活力。暴露 24 小时后通过流式细胞术测量细胞凋亡的诱导。使用实时定量聚合酶链反应测定暴露 1、3 和 21 天后成骨细胞中白细胞介素 6、白细胞介素 8、环氧化酶 2、半胱天冬酶 8、骨保护素和核因子 kappa-B 配体受体激活剂（RANKL）的表达。

结果

代表平台匹配组的金属离子浓度导致暴露 7 天内细胞活力降低（P<0.01）。刺激细胞显示出更高的早期细胞凋亡率（P<0.01）与未经处理的细胞相比。金属离子在暴露 1 天后以剂量依赖性方式上调白细胞介素 6、白细胞介素 8、环氧化酶 2 和 RANKL 的表达（P<0.05）。在含有平台匹配种植体-基台组合腐蚀产物的组中，上调更为明显。