Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China.
Enzyme Microb Technol. 2017 Nov;106:28-34. doi: 10.1016/j.enzmictec.2017.06.016. Epub 2017 Jun 29.
Cytochrome P450 enzymes are versatile biocatalysts with great potential in biotechnology. A new bacterial P450 was identified from the genome of Rhodococcus wratislaviensis NBRC 100605 and annotated as CYP108N7. The enzyme accepted the ferredoxin and ferredoxin reductase from spinach as surrogate redox partners for improved electron transfer efficiency. It was heterologous expressed in Escherichia coli together with the redox partners and a glucose dehydrogenase which supplied the reduced cofactor NADPH. The resulting whole-cell biocatalyst catalyzed a variety of reactions including sulfoxidation, epoxidation, hydroxylation, demethylation and dehalogenation. Remarkable stereoselectivity was observed in asymmetric sulfoxidation reaction, which could deliver chiral sulfoxides with >99% ee from thioanisole and derivatives.
细胞色素 P450 酶是多功能的生物催化剂，在生物技术中有很大的潜力。从 Rhodococcus wratislaviensis NBRC 100605 的基因组中鉴定出一种新的细菌 P450，并将其注释为 CYP108N7。该酶接受菠菜的铁氧还蛋白和铁氧还蛋白还原酶作为替代氧化还原伴侣，以提高电子转移效率。它与氧化还原伴侣和葡萄糖脱氢酶一起在大肠杆菌中异源表达，葡萄糖脱氢酶提供还原辅因子 NADPH。所得的全细胞生物催化剂可催化多种反应，包括磺氧化、环氧化、羟化、脱甲基和脱卤化。在不对称磺氧化反应中观察到显著的立体选择性，可从硫代茴香醚及其衍生物中以>99%ee 获得手性亚砜。
