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用于将质粒导入小鼠胚胎的金纳米线注射系统的研发。

Development of Au nanowire injector system to deliver plasmid into mouse embryo.

作者信息

Park Kkotchorong, Kim Keun Cheon, Lee Hyoban, Sung Yoori, Kang Mijeong, Lee Yun Mi, Ahn Ji Yeon, Lim Jeong Mook, Kang Taejoon, Kim Bongsoo, Lee Eun Ju

机构信息

Department of Chemistry, KAIST, Daejeon 34141, South Korea.

Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, South Korea.

出版信息

Data Brief. 2017 Jul 15;14:48-55. doi: 10.1016/j.dib.2017.07.017. eCollection 2017 Oct.

Abstract

In this data article, we developed a Au nanowire injector (Au NWI) for directly delivering plasmid into the 1-cell stage of the mouse embryos designed to successfully attach and detach the plasmid on the Au NWI, highly minimizing physical and chemical damage on the embryos. This data presents that a Au NWI system does not induce detrimental damages on development of embryos and efficiently express the green fluorescence protein in vitro. The data provided herein is in association with the research article related to reduce the occurrence of mosaicism by a Au NWI," Suppressing Mosaicism by Au Nanowire Injector-driven Direct Delivery of Plasmids into Mouse Embryos" (Park et al., 2017 [1]).

摘要

在本数据文章中,我们开发了一种金纳米线注射器(Au NWI),用于将质粒直接递送至小鼠胚胎的单细胞阶段,该设计旨在使质粒在金纳米线注射器上成功附着和分离,从而将对胚胎的物理和化学损伤降至最低。这些数据表明,金纳米线注射器系统不会对胚胎发育造成有害损伤,并且能在体外有效表达绿色荧光蛋白。本文提供的数据与一篇研究文章相关,该研究文章题为“通过金纳米线注射器驱动的质粒直接递送减少小鼠胚胎嵌合体的发生”(Park等人,2017 [1])。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad30/5567388/284272d4476b/gr1.jpg

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