重组tau蛋白的表达与分离
Expression and isolation of recombinant tau.
作者信息
Best Rebecca L, Chung Peter J, Benbow Sarah J, Savage April, LaPointe Nichole E, Safinya Cyrus R, Feinstein Stuart C
机构信息
Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States.
University of California, Santa Barbara, Santa Barbara, CA, United States.
出版信息
Methods Cell Biol. 2017;141:3-26. doi: 10.1016/bs.mcb.2017.06.001. Epub 2017 Jul 24.
In this chapter, we describe methods for the purification of both untagged and polyhistidine-tagged tau protein. These protocols utilize a bacterial expression system to produce the tau isoform of interest, followed by heat treatment and column chromatography to separate tau from impurities. These techniques yield a biochemically pure protein with which to pursue any number of questions regarding the mechanisms of tau action.
在本章中，我们描述了纯化未标记和多组氨酸标记的tau蛋白的方法。这些方案利用细菌表达系统来产生感兴趣的tau异构体，随后进行热处理和柱色谱法以将tau与杂质分离。这些技术产生了一种生化纯的蛋白质，可用于探讨任何数量有关tau作用机制的问题。
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