Inkaya Ahmet Cagkan, Demir Nazlim Aktug, Kolgelier Servet, Sumer Sua, Demir Lutfi Saltuk, Ural Onur, Pehlivan Fatma Seher, Aslan Mahmure, Arpaci Abdullah
Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Hacettepe University, Ankara Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Selçuk University, Konya Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Adiyaman University, Adiyaman Department of Public Health, Faculty of Medicine, Necmettin Erbakan University, Konya Department of Pathology, Adiyaman Education and Research Hospital, Adiyaman Department of Biochemistry, Adiyaman Education and Research Hospital, Adiyaman Department of Biochemistry, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey.
Medicine (Baltimore). 2017 Sep;96(36):e7547. doi: 10.1097/MD.0000000000007547.
High-mobility group box 1 (HMGB1), identified as an alarmin molecule, was shown to have a role in virus-triggered liver injury. We aimed to evaluate the association between serum levels of HMGB1 and liver fibrosis.
This cross-sectional case-control study included 189 chronic hepatitis B (CHB) patients and 51 healthy controls. All patients underwent liver biopsy and modified Knodell scoring system used to determine the fibrosis level in CHB patients. Serum HMGB1 levels were determined with enzyme-linked immunosorbent assay (ELISA).
Mean serum HMGB1 levels of patients (58.1 ± 54.7) were found to be higher than those of the control group (7.1 ± 4.3) (P = .001). HMGB1 levels of patients with advanced-stage fibrosis (stage 4 and 5) were detected to be higher than those of patients with early-stage fibrosis (stage 1-3). However, this difference was not statistically significant (P > .05). Albumin levels of fibrosis 3 and 4 patients were lower than fibrosis 1 and 2 patients. ALT, HBV DNA, and AFP levels of fibrosis 5 patients were significantly higher than fibrosis 1 and 2 patients, and their platelet and albumin levels are lower than fibrosis 1 and 2 patients (P < .001). In a logistic regression model, fibrosis levels were correlated with ALT values and inversely correlated with albumin levels.
In this study, we demonstrated that serum HMGB1 levels increase in the early course of liver injury and this increase is not correlated with severity of the liver damage.
高迁移率族蛋白B1（HMGB1）被鉴定为一种警报素分子，已被证明在病毒引发的肝损伤中起作用。我们旨在评估血清HMGB1水平与肝纤维化之间的关联。
这项横断面病例对照研究纳入了189例慢性乙型肝炎（CHB）患者和51名健康对照者。所有患者均接受了肝活检，并使用改良的Knodell评分系统来确定CHB患者的纤维化程度。采用酶联免疫吸附测定（ELISA）法测定血清HMGB1水平。
发现患者的平均血清HMGB1水平（58.1±54.7）高于对照组（7.1±4.3）（P = 0.001）。晚期纤维化（4期和5期）患者的HMGB1水平高于早期纤维化（1-3期）患者。然而，这种差异无统计学意义（P>0.05）。纤维化3期和4期患者的白蛋白水平低于纤维化1期和2期患者。纤维化5期患者的ALT、HBV DNA和AFP水平显著高于纤维化1期和2期患者，其血小板和白蛋白水平低于纤维化1期和2期患者（P<0.001）。在逻辑回归模型中，纤维化程度与ALT值相关，与白蛋白水平呈负相关。
在本研究中，我们证明血清HMGB1水平在肝损伤早期升高，且这种升高与肝损伤的严重程度无关。
Zotero 插件