丝状病毒的反向遗传学。

Reverse Genetics of Filoviruses.

机构信息

Friedrich-Loeffler-Institut, Südufer 10, 17493, Greifswald-Insel Riems, Germany.

Integrated Research Facility at Fort Detrick (IRF-Frederick), Division of Clinical Research (DCR), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), B-8200 Research Plaza, Fort Detrick, Frederick, MD, 21702, USA.

出版信息

Curr Top Microbiol Immunol. 2017;411:421-445. doi: 10.1007/82_2017_55.

DOI:10.1007/82_2017_55

PMID:28918537

Abstract

Reverse genetics systems are used for the generation of recombinant viruses. For filoviruses, this technology has been available for more than 15 years and has been used to investigate questions regarding the molecular biology, pathogenicity, and host adaptation determinants of these viruses. Further, reporter-expressing, recombinant viruses are increasingly used as tools for screening for and characterization of candidate medical countermeasures. Thus, reverse genetics systems represent powerful research tools. Here we provide an overview of available reverse genetics systems for the generation of recombinant filoviruses, potential applications, and the achievements that have been made using these systems.

摘要

反向遗传学系统用于重组病毒的产生。对于丝状病毒，这项技术已经存在了 15 年以上，并且已被用于研究这些病毒的分子生物学、致病性和宿主适应性决定因素等问题。此外，表达报告基因的重组病毒越来越多地被用作筛选和鉴定候选医疗对策的工具。因此，反向遗传学系统代表了强大的研究工具。本文概述了用于产生重组丝状病毒的现有反向遗传学系统、潜在的应用以及使用这些系统取得的成就。