Delgado-Altamirano Ronna, Monzote Lianet, Piñón-Tápanes Abel, Vibrans Heike, Rivero-Cruz J Fausto, Ibarra-Alvarado César, Rojas-Molina Alejandra
Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010, Querétaro, Mexico.
Laboratorio de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, 76010, Querétaro, Mexico.
Heliyon. 2017 Sep 8;3(9):e00394. doi: 10.1016/j.heliyon.2017.e00394. eCollection 2017 Sep.
To evaluate the anti-leishmanial activity and cytotoxicity of aqueous and organic extracts of ten plants used in Mexican traditional medicine as anti-parasitics.
For the organic extracts, plant material was macerated in dichloromethane (CHCl) and dichloromethane/methanol (CHCl/MeOH) (1:1) during two weeks; the aqueous extracts were prepared by infusion. The extracts were tested against promastigotes and intracellular amastigotes of . The cytotoxicity was assayed in parallel on peritoneal macrophages of BALB/c mice.
Four of the thirty extracts tested were active and selective against promastigotes: (CHCl and CHCl/MeOH), (CHCl) and (aqueous). These extracts had a median inhibitory concentration (IC) against intracellular amastigotes under 50 μg/mL and a selectivity index (SI) higher than 5, which indicates that they constitute valuable candidates to obtain secondary metabolites with leishmanicidal activity.
The results derived from this study indicate that and might provide interesting new leads for the development of antileishmanial drugs.
评估墨西哥传统医学中用作抗寄生虫药的10种植物的水提取物和有机提取物的抗利什曼原虫活性及细胞毒性。
对于有机提取物,将植物材料在二氯甲烷(CHCl)和二氯甲烷/甲醇(CHCl/MeOH)(1:1)中浸渍两周;水提取物通过浸泡制备。提取物针对杜氏利什曼原虫前鞭毛体和细胞内无鞭毛体进行测试。同时在BALB/c小鼠的腹腔巨噬细胞上测定细胞毒性。
所测试的30种提取物中有4种对杜氏利什曼原虫前鞭毛体具有活性且具有选择性:(CHCl和CHCl/MeOH)、(CHCl)和(水提取物)。这些提取物对细胞内无鞭毛体的半数抑制浓度(IC)低于50μg/mL,选择性指数(SI)高于5,这表明它们是获得具有杀利什曼原虫活性的次生代谢物的有价值候选物。
本研究结果表明,[植物名称1]和[植物名称2]可能为抗利什曼原虫药物的开发提供有趣的新线索。