Hoffman Jessica F, Vergara Vernieda B, Mog Steven R, Kalinich John F
Internal Contamination and Metal Toxicity Program, Armed Forces Radiobiology Research Institute, Uniformed Services University, Bethesda, MD 20889, USA.
Office of Food Additive Safety (OFAS), CFSAN/FDA/DHHS, College Park, MD 20740, USA.
Toxics. 2017 Oct 11;5(4):25. doi: 10.3390/toxics5040025.
Hydrophobic sand is a relatively new method of urine collection in the rodent, comparable to the established method using a metabolic cage. Urine samples are often used in rodent research, especially for biomarkers of health changes after internal contamination from embedded metals, such as in a model of a military shrapnel wound. However, little research has been done on the potential interference of hydrophobic sand with urine metal concentrations either by contamination from the sand particulate, or adsorption of metals from the urine. We compare urine collected from rats using the metabolic cage method and the hydrophobic sand method for differences in metal concentration of common urinary metals, and examine physical properties of the sand material for potential sources of contamination. We found minimal risk of internal contamination of the rat by hydrophobic sand, and no interference of the sand with several common metals of interest (cobalt, strontium, copper, and manganese), although we advise caution in studies of aluminum in urine.
疏水砂是一种相对较新的啮齿动物尿液收集方法，可与使用代谢笼的既定方法相媲美。尿液样本常用于啮齿动物研究，特别是用于研究嵌入金属内部污染后健康变化的生物标志物，例如在军事弹片伤模型中。然而，关于疏水砂颗粒污染或尿液中金属吸附对尿液金属浓度的潜在干扰，目前研究较少。我们比较了使用代谢笼法和疏水砂法从大鼠收集的尿液中常见尿金属的浓度差异，并检查了砂材料的物理性质以寻找潜在污染源。我们发现疏水砂对大鼠内部污染的风险极小，并且该砂对几种常见的感兴趣金属（钴、锶、铜和锰）没有干扰，不过我们建议在尿液铝研究中要谨慎。
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