GC-MS 分析蛹虫草菌丝体不同发酵产物中的挥发性成分。
GC-MS Profiling of Volatile Components in Different Fermentation Products of Cordyceps Sinensis Mycelia.
机构信息
School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Molecules. 2017 Oct 24;22(10):1800. doi: 10.3390/molecules22101800.
The fermentation products of () mycelia are sustainable substitutes for natural . However, the volatile compositions of the commercial products are still unclear. In this paper, we have developed a simultaneous distillation-extraction (SDE) and gas chromatography-mass spectrometry (GC-MS) method for the profiling of volatile components in five fermentation products. A total of 64, 39, 56, 52, and 44 components were identified in the essential oils of Jinshuibao capsule (JSBC), Bailing capsule (BLC), Zhiling capsule (ZLC), Ningxinbao capsule (NXBC), and Xinganbao capsule (XGBC), respectively. 5,6-Dihydro-6-pentyl-2H-pyran-2-one (massoia lactone) was first discovered as the dominant component in JSBC volatiles. Fatty acids including palmitic acid (C16:0) and linoleic acid (C18:2) were also found to be major volatile compositions of the fermentation products. The multivariate partial least squares-discriminant analysis (PLS-DA) showed a clear discrimination among the different commercial products as well as the counterfeits. This study may provide further chemical evidences for the quality evaluation of the fermentation products of mycelia.
（）菌丝体的发酵产物是天然产物的可持续替代品。然而，商业产品的挥发性成分仍不清楚。在本文中，我们开发了一种同时蒸馏萃取（SDE）和气相色谱-质谱联用（GC-MS）方法，用于分析五种发酵产物中挥发性成分的分布。在金水宝胶囊（JSBC）、百灵胶囊（BLC）、智灵胶囊（ZLC）、宁心宝胶囊（NXBC）和兴安宝胶囊（XGBC）的精油中分别鉴定出 64、39、56、52 和 44 种成分。首次发现 5,6-二氢-6-戊基-2H-吡喃-2-酮（massoia 内酯）是 JSBC 挥发物中的主要成分。还发现脂肪酸，包括棕榈酸（C16:0）和亚油酸（C18:2）也是发酵产物的主要挥发性成分。多变量偏最小二乘判别分析（PLS-DA）表明不同商业产品以及假冒产品之间存在明显的区分。这项研究可能为菌丝体发酵产物的质量评价提供进一步的化学证据。
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