Zhong Chuanqing, Zhang Chao, Fu Jiafang, Chen Wenbing, Jiang Tianyi, Cao Guangxiang
a School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, People's Republic of China.
b Shandong Medicinal Biotechnology Centre, Shandong Academy of Medical Sciences, Jinan 250062, People's Republic of China.
Can J Microbiol. 2018 Jan;64(1):87-90. doi: 10.1139/cjm-2017-0475. Epub 2017 Oct 26.
Enterobacter cloacae strain R11 is a multidrug-resistant bacterium isolated from sewage water near a swine feedlot in China. Strain R11 can survive in medium containing up to 192 μg/mL polymyxin E, indicating a tolerance for this antibiotic that is significantly higher than that reported for other gram-negative bacteria. In this study, conjugation experiments showed that partial polymyxin E resistance could be transferred from strain R11 to Escherichia coli strain 25922, revealing that some genes related to polymyxin E resistance are plasmid-based. The complete genome sequence of this strain was determined, yielding a total of 4 993 008 bp (G+C content, 53.15%) and 4908 genes for the circular chromosome and 4 circular plasmids. Genome analysis revealed a total of 73 putative antibiotic resistance genes, including several polymyxin E resistance genes and genes potentially involved in multidrug resistance. These data provide insights into the genetic basis of the polymyxin E resistance and multidrug resistance of E. cloacae.
阴沟肠杆菌R11菌株是从中国一个养猪场附近的污水中分离出的一种多重耐药细菌。R11菌株能够在含有高达192μg/mL多粘菌素E的培养基中存活，这表明其对这种抗生素的耐受性显著高于其他革兰氏阴性菌。在本研究中，接合实验表明部分多粘菌素E耐药性可从R11菌株转移至大肠杆菌25922菌株，这表明一些与多粘菌素E耐药性相关的基因是基于质粒的。测定了该菌株的完整基因组序列，环状染色体和4个环状质粒的总长度为4993008bp（G+C含量为53.15%），共有4908个基因。基因组分析共发现73个推定的抗生素耐药基因，包括几个多粘菌素E耐药基因和可能参与多重耐药的基因。这些数据为阴沟肠杆菌对多粘菌素E的耐药性和多重耐药性的遗传基础提供了见解。
