State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan, 650091, P. R. China.
Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, 650500, P. R. China.
J Microbiol. 2017 Nov;55(11):885-891. doi: 10.1007/s12275-017-7169-x. Epub 2017 Oct 27.
Reactive oxygen species (ROS) produced by NADPH oxidases can serve as signaling molecules to regulate a variety of physiological processes in multi-cellular organisms. In the nematophagous fungus Arthrobotrys oligospora, we found that ROS were produced during conidial germination, hyphal extension, and trap formation in the presence of nematodes. Generation of an AoNoxA knockout strain demonstrated the crucial role of NADPH oxidase in the production of ROS in A. oligospora, with trap formation impaired in the AoNoxA mutant, even in the presence of the nematode host. In addition, the expression of virulence factor serine protease P186 was up-regulated in the wild-type strain, but not in the mutant strain, in the presence of Caenorhabditis elegans. These results indicate that ROS derived from AoNoxA are essential for full virulence of A. oligospora in nematodes.
活性氧（ROS）由 NADPH 氧化酶产生，可以作为信号分子来调节多细胞生物中的各种生理过程。在食线虫真菌节丛孢菌中，我们发现 ROS 在孢子萌发、菌丝延伸和在存在线虫的情况下形成陷阱时产生。AoNoxA 敲除株的生成表明 NADPH 氧化酶在 AoNoxA 中 ROS 的产生中起关键作用，即使存在线虫宿主，AoNoxA 突变体中的陷阱形成也受到损害。此外，在秀丽隐杆线虫存在的情况下，毒力因子丝氨酸蛋白酶 P186 的表达在野生型菌株中上调，但在突变体菌株中没有上调。这些结果表明，AoNoxA 衍生的 ROS 对节丛孢菌在线虫中的完全毒力是必不可少的。
