通过环境条件模拟杜氏盐藻生长动力学和优化生物活性化合物的生产。
Modelling Tetraselmis sp. growth-kinetics and optimizing bioactive-compound production through environmental conditions.
机构信息
Unité de Biotechnologie des Algues, Biological Engineering Department, National School of Engineers of Sfax, University of Sfax, Sfax, Tunisia.
Centre de Biophysique Moléculaire CBM - CNRS, Université d'Orléans, Orleans, France.
出版信息
Bioresour Technol. 2018 Feb;249:510-518. doi: 10.1016/j.biortech.2017.10.028. Epub 2017 Oct 10.
The aim of this study is to predict Tetraselmis cells growth-kinetic and to induce the synthesis of bioactive compounds (chlorophylls, carotenoids and starch) with high potential for biotechnological applications. Using the statistical criteria, the Baranyi-Roberts model has been selected to estimate the microalgae growth-kinetic values. The simultaneous effects of salinity, light intensity and pH of culture medium were investigated to maximize the production of total chlorophylls, carotenoids and starch. The optimal culture conditions for the production of these compounds were found using Box-Behnken Design. Results have shown that total chlorophyll and carotenoids were attained 21.6mg·gDW and 0.042mg·gDW, respectively. In addition, the highest starch content of 0.624g·gDW has been obtained at neutral pH with high irradiance (182μmolphotonsm s) and low salinity (20). A highly correlation (R = 0.884) has been found between the gravimetric and flow cytometric measurements of chlorophyll content.
本研究旨在预测球等鞭金藻的生长动力学，并诱导具有高生物技术应用潜力的生物活性化合物（叶绿素、类胡萝卜素和淀粉）的合成。使用统计标准，选择了 Baranyi-Roberts 模型来估算微藻的生长动力学值。研究了盐度、光照强度和培养液 pH 值的同时作用，以最大限度地提高总叶绿素、类胡萝卜素和淀粉的产量。使用 Box-Behnken 设计找到了这些化合物生产的最佳培养条件。结果表明，总叶绿素和类胡萝卜素分别达到 21.6mg·gDW 和 0.042mg·gDW。此外，在中性 pH 值、高光强（182μmolphotonsm·s）和低盐度（20）条件下，淀粉含量达到了 0.624g·gDW 的最高值。叶绿素含量的重量法和流式细胞术测量之间存在高度相关性（R=0.884）。
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