Department of Chemical Engineering & Materials Science, University of California, Irvine, CA, 92697, USA.
Department of Biomedical Engineering, University of California, Irvine, CA, 92697, USA.
Adv Healthc Mater. 2017 Dec;6(24). doi: 10.1002/adhm.201700707. Epub 2017 Oct 30.
Leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1) is an inhibitory receptor broadly expressed on immune cells, with its ligands residing within the extracellular matrix protein collagen. In this study, surfaces are modified with a LAIR-1 ligand peptide (LP), and it is observed that macrophages cultured on LAIR-1 LP-conjugated surfaces exhibit significantly reduced secretion of inflammatory cytokines in response to proinflammatory stimuli that reflect an injured environment. These downregulated mediators include TNF-α, MIP-1α, MIP-1β, MIP-2, RANTES, and MIG. Knockdown of LAIR-1 using siRNA abrogates this inhibition of cytokine secretion, supporting the specificity of the inhibitory effect to this receptor. These results are the first to demonstrate that integration of LAIR-1 ligands with biomaterials could suppress inflammatory responses.
白细胞相关免疫球蛋白样受体-1（LAIR-1）是一种广泛表达于免疫细胞上的抑制性受体，其配体位于细胞外基质蛋白胶原内。在本研究中，通过修饰表面使其结合 LAIR-1 配体肽（LP），结果发现，在 LAIR-1 LP 偶联表面培养的巨噬细胞对反映损伤环境的促炎刺激物的炎症细胞因子分泌显著减少。这些下调的介质包括 TNF-α、MIP-1α、MIP-1β、MIP-2、RANTES 和 MIG。使用 siRNA 敲低 LAIR-1 可消除细胞因子分泌的这种抑制作用，支持这种抑制作用对该受体的特异性。这些结果首次表明，将 LAIR-1 配体与生物材料整合可以抑制炎症反应。
