RGS4 和 P16 表达与小儿肾母细胞瘤的相关性及其对预后的意义。

Correlation of RGS4 and P16 expressions with pediatric nephroblastoma and its significance on prognosis.

机构信息

Department of Pediatrics, Jining No. 1 People's Hospital, Jining, Shandong Province, China.

出版信息

Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4577-4583.

Abstract

OBJECTIVE

To investigate the correlation of regulator of G-protein signaling 4 (RGS4) and P16 expressions with pediatric nephroblastoma and its significance on prognosis.

PATIENTS AND METHODS

A total of 80 children with primary nephroblastoma who underwent surgical resection in our hospital from March 2009 to March 2012 were selected as objects of study. The expressions of RGS4 and P16 in cancer and cancer-adjacent tissues of patients were detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry. All patients were followed up for 5 years. The relationship of RGS4 and P16 to nephroblastoma staging and patients' prognosis was analyzed.

RESULTS

The results of RT-PCR and Western blot displayed that the expressions of RGS4, P16 mRNA and protein in cancer tissue, were significantly lower than those in cancer-adjacent tissue (p < 0.05). Immunohistochemistry result revealed that the positive rates of RGS4 and P16 in cancer tissue were distinctly lower than those in cancer-adjacent tissue (76.54% vs. 34.32%, p < 0.05). RGS4 and P16 protein expressions were not associated with tumor, node metastasis (TNM) and pathological typing of nephroblastoma, but they were lowly expressed in patients with high metastasis (p < 0.05). The expressions of RGS4 and P16 were absent in pediatric nephroblastoma, and overall survival (OS) was significantly reduced (p < 0.05).

CONCLUSIONS

The absence of RGS4 and P16 in pediatric nephroblastoma tissue is correlated with poor prognosis of patients. RGS4 and P16 are of significance for the prognosis of pediatric nephroblastoma.

摘要

目的

研究 G 蛋白信号调节因子 4（RGS4）和 P16 的表达与小儿肾母细胞瘤的相关性及其对预后的意义。

方法

选取 2009 年 3 月至 2012 年 3 月我院收治的 80 例原发性肾母细胞瘤患儿作为研究对象，采用逆转录-聚合酶链反应（RT-PCR）、Western blot 和免疫组化法检测患者癌组织及癌旁组织中 RGS4 和 P16 的表达，所有患者均随访 5 年，分析 RGS4 和 P16 与肾母细胞瘤分期及患者预后的关系。

结果

RT-PCR 和 Western blot 结果显示，癌组织中 RGS4、P16mRNA 及蛋白表达均显著低于癌旁组织（p<0.05）；免疫组化结果显示，癌组织中 RGS4 和 P16 阳性率均显著低于癌旁组织（76.54%比 34.32%，p<0.05）。RGS4 和 P16 蛋白表达与肿瘤、淋巴结转移（TNM）和肾母细胞瘤病理分型无关，但在转移率高的患者中表达较低（p<0.05）。小儿肾母细胞瘤组织中 RGS4 和 P16 表达缺失，总生存率（OS）显著降低（p<0.05）。