Department of Food and Nutrition, Sookmyung Women's University, Seoul 04310, Korea.
Risk Analysis Research Center, Sookmyung Women's University, Seoul 04310, Korea.
Int J Environ Res Public Health. 2017 Nov 17;14(11):1400. doi: 10.3390/ijerph14111400.
This study examined antibiotic susceptibility, genetic diversity, and characteristics of virulence genes in isolates from poultry. Chicken ( = 152) and duck ( = 154) samples were collected from 18 wet markets in Korea. spp. isolated from the carcasses were identified by PCR. The isolated colonies were analyzed for antibiotic susceptibility to chloramphenicol, amikacin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, and enrofloxacin. The isolates were also used to analyze genetic diversity using the DiversiLab system and were tested for the presence of cytolethal distending toxin () genes. spp. were isolated from 45 poultry samples out of 306 poultry samples (14.7%) and the average levels of contamination were 22.0 CFU/g and 366.1 CFU/g in chicken and duck samples, respectively. Moreover, more than 90% of the isolates showed resistance to nalidixic acid and ciprofloxacin. Genetic correlation analysis showed greater than 95% similarity between 84.4% of the isolates, and three genes (, , and ) were present in 71.1% of isolates. These results indicate that contamination should be decreased to prevent and treat foodborne illness.
本研究检测了来自家禽的 分离株的抗生素敏感性、遗传多样性和毒力基因特征。从韩国的 18 个湿市场采集了鸡（=152）和鸭（=154）样本。通过 PCR 鉴定从胴体中分离出的 。对分离出的菌落进行了氯霉素、阿米卡星、红霉素、四环素、环丙沙星、萘啶酸和恩诺沙星的抗生素敏感性分析。还使用 DiversiLab 系统分析了分离株的遗传多样性，并检测了细胞扩张毒素 () 基因的存在。从 306 个家禽样本中的 45 个样本中分离出了 ，鸡和鸭样本中 的平均污染水平分别为 22.0 CFU/g 和 366.1 CFU/g。此外，超过 90%的分离株对萘啶酸和环丙沙星表现出耐药性。遗传相关分析显示，84.4%的分离株之间的相似度大于 95%，71.1%的分离株中存在 3 个 基因（， 和 ）。这些结果表明，应减少 的污染，以预防和治疗食源性疾病。
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