泰国、老挝和缅甸人群中 和 卵的分子鉴定。
Molecular Identification of and Eggs in Human Populations from Thailand, Lao PDR, and Myanmar.
机构信息
Department of Parasitology, Faculty of Medicine, and Research and Diagnostic Center for Infectious Diseases, Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand.
Faculty of Medicine, Maha Sarakram University, Maha Sarakram, Thailand.
出版信息
Am J Trop Med Hyg. 2018 Jan;98(1):39-44. doi: 10.4269/ajtmh.17-0651.
is a soil-transmitted helminth infecting human populations globally. Human cases caused by and have also been reported. Molecular identifications of species infecting human populations in Lao PDR and Myanmar are lacking. Here, we explored molecular data obtained from eggs recovered from human fecal samples from these countries and compared these with new and existing data from Thailand. Nuclear ribosomal DNA (18S and ITS2) sequences were amplified from eggs and sequenced. Forty-one samples showed 99-100% similarity in their 18S sequences to published sequences of and one sample showed 99% similarity to a sequence of . Similarly, 41 samples showed 92-100% similarity in their ITS2 sequences to published sequences of and one sample showed 94-97% similarity to sequences of . This study is the first molecular confirmation of human infection with in northeast Thailand and the first molecular confirmation of the species of infecting humans in Lao PDR and Myanmar.
是一种感染全球人类的土壤传播性蠕虫。也有人类感染 和 的病例报告。缺乏老挝人民民主共和国和缅甸感染人类的 物种的分子鉴定。在这里，我们探索了从这些国家人类粪便样本中回收的 卵获得的分子数据，并将其与来自泰国的新的和现有的数据进行了比较。从 卵中扩增了核核糖体 DNA（18S 和 ITS2）序列并进行了测序。41 个样本在 18S 序列上与已发表的 序列具有 99-100%的相似性，一个样本与 的序列具有 99%的相似性。同样，41 个样本在 ITS2 序列上与已发表的 序列具有 92-100%的相似性，一个样本与 的序列具有 94-97%的相似性。这项研究首次在分子水平上确认了泰国东北部地区人类感染 的情况，也是首次在分子水平上确认了老挝人民民主共和国和缅甸感染人类的 物种。
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