Farhan Yasmine, Smith Jocelyn L, Schaafsma Arthur W
Department of Plant Agriculture, Ridgetown Campus, University of Guelph, Ridgetown, ON, Canada.
J Econ Entomol. 2018 Feb 9;111(1):65-71. doi: 10.1093/jee/tox296.
Striacosta albicosta (Smith; Lepidoptera: Noctuidae) is a pest of corn (Zea mays L.), which has recently expanded its range into Ontario, Canada. Genetically modified corn expressing Vip3A insecticidal protein from Bacillus thuringiensis is a biotechnological option for the control of S. albicosta. To support an insect resistance management program, we conducted a study of baseline susceptibility of 10-field collected S. albicosta populations in Ontario, Canada to Vip3A before widespread commercial adoption. Neonates were exposed to artificial diet overlaid with Vip3A. The LC50 ranged from 22.7 to 53.5 ng Vip3A cm-2. The EC50 ranged from 11.4 to 30.2 ng Vip3A cm-2. There was low inter-population variation in susceptibility to Vip3A, which we believe represents the natural geographical variation in response and not variation caused by previous exposure to selection pressure of the Vip3A protein.
白边黏虫（史密斯；鳞翅目：夜蛾科）是玉米（玉米属）的一种害虫，其分布范围最近已扩展至加拿大安大略省。表达来自苏云金芽孢杆菌的Vip3A杀虫蛋白的转基因玉米是控制白边黏虫的一种生物技术手段。为支持抗虫管理计划，在广泛商业应用之前，我们对加拿大安大略省10个田间采集的白边黏虫种群对Vip3A的基线敏感性进行了研究。将初孵幼虫暴露于覆盖有Vip3A的人工饲料上。半数致死浓度（LC50）范围为22.7至53.5纳克Vip3A每平方厘米。半数有效浓度（EC50）范围为11.4至30.2纳克Vip3A每平方厘米。种群间对Vip3A的敏感性差异较低，我们认为这代表了自然地理响应差异，而非先前接触Vip3A蛋白选择压力导致的差异。
