Lysobacter tongrenensis sp. nov., isolated from soil of a manganese factory.

A Gram-staining negative, aerobic, non-motile, rod-shaped bacterial strain, designated YS-37, was isolated from soil in a manganese factory, People's Republic of China. Based on16S rRNA gene sequence analysis, strain YS-37 was most closely related to Lysobacter pocheonensis Gsoil 193 (97.0%), Lysobacter dokdonensis DS-58 (96.0%) and Lysobacter daecheongensis Dae08 (95.8%) and grouped together with L. pocheonensis Gsoil 193 and Lysobacter dokdonensis DS-58. The DNA-DNA hybridization value between strain YS-37 and L. pocheonensis KCTC 12624 was 43.3% (± 1). The major respiratory quinone of strain YS-37 was ubiquinone-8, and the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phospholipid, phosphatidylmethylethaolamine and two unknown lipids. Its major cellular fatty acids (> 5%) were iso-C, iso-C ω9c, iso-C, iso-C 3-OH and iso-C and the G + C content of the genomic DNA was 67.1 mol%. Strain YS-37 also showed some biophysical and biochemical differences with the related strains, especially in hydrolysis of casein. The results demonstrated that strain YS-37 belongs to genus Lysobacter and represents a novel Lysobacter species for which the name Lysobacter tongrenensis sp. nov. is proposed. The type strain is YS-37 (= CCTCC AB 2016052 = KCTC 52206).