巴黎花叶坏死病毒的全基因组序列，马铃薯Y病毒属的一个独特成员。
Complete genome sequence of Paris mosaic necrosis virus, a distinct member of the genus Potyvirus.
作者信息
Lan Pingxiu, Zhao Jurun, Zhou Yalin, Li Yueyue, Shen Dingcai, Liao Qinchan, Li Ruhui, Li Fan
机构信息
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China.
College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
出版信息
Arch Virol. 2018 Mar;163(3):787-790. doi: 10.1007/s00705-017-3649-x. Epub 2017 Nov 29.
The complete genomic sequence of a novel potyvirus was determined from Paris polyphylla var. yunnanensis. Its genomic RNA consists of 9,660 nucleotides (nt) excluding the 3'-terminal poly (A) tail, containing the typical open reading frame (ORF) of potyviruses and encoding a putative large polyprotein of 3030 amino acids. The virus shares 53.9-70.1% nt sequence identity and 43.9-73.2% amino acid sequence identity with other viruses classified within the genus Potyvirus. Proteolytic cleavage sites and conserved motifs of the potyviruses were identified in the polyprotein and within individual proteins. Phylogenetic analysis indicated that the virus is most closely related to members of the BCMV subgroup. The results suggest that the virus should be classified as a novel species within the genus Potyvirus, which we tentatively name "Paris mosaic necrosis virus".
从滇重楼中确定了一种新型马铃薯Y病毒的完整基因组序列。其基因组RNA由9660个核苷酸（nt）组成，不包括3'-末端的聚（A）尾，含有马铃薯Y病毒典型的开放阅读框（ORF），并编码一个推定的由3030个氨基酸组成的大的多聚蛋白。该病毒与马铃薯Y病毒属内其他病毒的核苷酸序列同一性为53.9-70.1%，氨基酸序列同一性为43.9-73.2%。在多聚蛋白和单个蛋白质中鉴定出了马铃薯Y病毒的蛋白水解切割位点和保守基序。系统发育分析表明，该病毒与蚕豆普通花叶病毒亚组的成员关系最为密切。结果表明，该病毒应被归类为马铃薯Y病毒属内的一个新种，我们暂定其名为“滇重楼花叶坏死病毒”。
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