Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, Oxford University.
Chinese Academy of Medical Sciences-Oxford Center for Translational Immunology, Nuffield Department of Medicine, Oxford University, United Kingdom.
J Infect Dis. 2018 Jan 4;217(2):257-262. doi: 10.1093/infdis/jix512.
Interferon-induced transmembrane 3 (IFITM3) is known to restrict the entry of a range of enveloped viruses. The single nucleotide polymorphism rs12252-C within IFITM3 has been shown to be associated with severe influenza A virus infection. It has been suggested that rs12252-C results in expression of a truncated IFITM3 protein lacking the first 21 amino acids. By performing high-throughput RNA sequencing on primary dendritic cells and peripheral blood mononuclear cells isolated from pandemic H1N1 influenza and human immunodeficiency virus-1 (HIV-1) infected patients we show that full-length IFITM3 mRNA is dominantly expressed (>99%) across all rs12252 genotypes. Full-length IFITM3 protein can be detected in all genotypes.
干扰素诱导跨膜蛋白 3（IFITM3）已知可限制多种包膜病毒的进入。IFITM3 中的单核苷酸多态性 rs12252-C 已被证明与严重的甲型流感病毒感染有关。有人认为 rs12252-C 导致表达缺少前 21 个氨基酸的截断 IFITM3 蛋白。通过对大流行性 H1N1 流感和人类免疫缺陷病毒-1（HIV-1）感染患者分离的原代树突状细胞和外周血单核细胞进行高通量 RNA 测序，我们表明全长 IFITM3 mRNA 在所有 rs12252 基因型中均占主导地位（>99%）。所有基因型都可以检测到全长 IFITM3 蛋白。
