一个 CBL 相互作用蛋白激酶 TaCIPK27 在转基因拟南芥中赋予耐旱性和对外源 ABA 的敏感性。

A CBL-interacting protein kinase TaCIPK27 confers drought tolerance and exogenous ABA sensitivity in transgenic Arabidopsis.

机构信息

The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

出版信息

Plant Physiol Biochem. 2018 Feb;123:103-113. doi: 10.1016/j.plaphy.2017.11.019. Epub 2017 Dec 1.

DOI:10.1016/j.plaphy.2017.11.019

PMID:29227949

Abstract

Drought is one of the major environmental stresses to plants. The calcium sensor, calcineurin B-like (CBL) proteins, and their interacting protein kinases (CIPK) play important roles in responding to abiotic stresses. In this study, we functionally characterized a CIPK gene from Triticum aestivum designated TaCIPK27. The transcriptional levels of TaCIPK27 were increased both in roots and leaves after treatment with polyethylene glycol 8000, abscisic acid and HO. Besides, TaCIPK27 interacted with AtCBL1, AtCBL3, AtCBL4, AtCBL5 and AtCBL9 in yeast two-hybrid assays. Ectopic overexpression of TaCIPK27 positively regulates drought tolerance in transgenic Arabidopsis compared with controls, which was demonstrated by seed germination and survival rates experiments, as well as the detection of physiological indices including ion leakage, malonic dialdehyde and HO contents and antioxidant enzyme activities under normal and drought conditions. Moreover, higher concentration of endogenous abscisic acid was detected under drought in TaCIPK27 transgenic plants. In addition, TaCIPK27 transgenic plants were more sensitive to exogenous abscisic acid treatment at seed germination and seedling stage. The expression levels of somedrought stress and abscisic acid related genes were up-regulated in TaCIPK27 transgenic plants. The results suggest that TaCIPK27 functions as a positive regulator under drought partly in an ABA-dependent pathway.

摘要

干旱是植物面临的主要环境胁迫之一。钙调磷酸酶 B 类似蛋白（CBL）及其相互作用的蛋白激酶（CIPK）在响应非生物胁迫方面发挥着重要作用。本研究从普通小麦中鉴定并功能分析了一个 CIPK 基因 TaCIPK27。PEG8000、ABA 和 H2O2 处理后，TaCIPK27 在根和叶中的转录水平均升高。此外，在酵母双杂交试验中，TaCIPK27 与 AtCBL1、AtCBL3、AtCBL4、AtCBL5 和 AtCBL9 互作。与对照相比，TaCIPK27 的过表达在拟南芥中正向调控干旱胁迫耐受性，这可通过种子萌发和存活率实验以及正常和干旱条件下的生理指标检测（如离子渗漏、丙二醛和 H2O2 含量以及抗氧化酶活性）得到证明。此外，在干旱胁迫下，TaCIPK27 转基因植株中检测到更高浓度的内源性脱落酸。此外，在种子萌发和幼苗期，TaCIPK27 转基因植株对外源脱落酸处理更敏感。在 TaCIPK27 转基因植株中，一些干旱胁迫和脱落酸相关基因的表达水平上调。这些结果表明，TaCIPK27 作为一个正调控因子，部分通过 ABA 依赖途径参与干旱胁迫响应。