18F-FDG PET 生物标志物有助于检测不可逆电穿孔治疗早期的代谢反应，并预测大鼠肝肿瘤模型的治疗效果。
F-FDG PET Biomarkers Help Detect Early Metabolic Response to Irreversible Electroporation and Predict Therapeutic Outcomes in a Rat Liver Tumor Model.
机构信息
From the Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N Michigan Ave, 16th Floor, Chicago, IL, 60611 (X.W.). Department of Radiology, Feinberg School of Medicine, Northwestern University, 737 N Michigan Ave, 16th Floor, Chicago, IL, 60611 (X.W., Z.S., T.L., M.F., D.P., J.S., C.S., B.W., N.S., S.G., Q.M., A.C.G., K.L., R.J.L., R.S., V.Y., A.C.L., Z.Z.); Department of Radiology, Shanghai General Hospital, Shanghai Jiaotong University, Shanghai, People's Republic of China (X.W.); Department of Radiology, Tianjin Xiqing Hospital, Tianjin, China (Z.S.);.Department of Orthopedics, Qilu Hospital, Shandong University, Jinan, Shandong, China (C.S.); Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China (B.W.); Department of Interventional Radiology, Hunan Cancer Hospital, Changsha, Hunan, China (S.G.); Department of Radiology, Southwest Hospital, Chongqing, China (J.W.); and Robert H. Lurie Comprehensive Cancer Center, Chicago, Ill (R.S., V.Y., A.C.L., Z.Z.).
出版信息
Radiology. 2018 Apr;287(1):137-145. doi: 10.1148/radiol.2017170920. Epub 2017 Dec 12.
Purpose To test the hypothesis that biomarkers of fluorine 18 (F) fluorodeoxyglucose (FDG) positron emission tomography (PET) can be used for the early detection of therapeutic response to irreversible electroporation (IRE) of liver tumor in a rodent liver tumor model. Materials and Methods The institutional animal care and use committee approved this study. Rats were inoculated with McA-RH7777 liver tumor cells in the left median and left lateral lobes. Tumors were allowed to grow for 7 days to reach a size typically at least 5 mm in longest diameter, as verified with magnetic resonance (MR) imaging. IRE electrodes were inserted, and eight 100-μsec, 2000-V pulses were applied to ablate the tumor tissue in the left median lobe. Tumor in the left lateral lobe served as a control in each animal. PET/computed tomography (CT) and MR imaging measurements were performed at baseline and 3 days after IRE for each animal. Additional MR imaging measurements were obtained 14 days after IRE. After 14-day follow-up MR imaging, rats were euthanized and tumors harvested for hematoxylin-eosin, CD34, and caspase-3 staining. Change in the maximum standardized uptake value (ΔSUV) was calculated 3 days after IRE. The maximum lesion diameter change (ΔD) was measured 14 days after IRE by using axial T2-weighted imaging. ΔSUV and ΔD were compared. The apoptosis index was calculated by using caspase-3-stained slices of apoptotic tumor cells. Pearson correlation coefficients were calculated to assess the relationship between ΔSUV at 3 days and ΔD (or apoptosis index) at 14 days after IRE treatment. Results ΔSUV ΔD, and apoptosis index significantly differed between treated and untreated tumors (P < .001 for all). In treated tumors, there was a strong correlation between ΔSUV 3 days after IRE and ΔD 14 days after IRE (R = 0.66, P = .01) and between ΔSUV 3 days after IRE and apoptosis index 14 days after IRE (R = 0.57, P = .04). Conclusion F-FDG PET imaging biomarkers can be used for the early detection of therapeutic response to IRE treatment of liver tumors in a rodent model. RSNA, 2017.
目的 检验氟 18（F）氟脱氧葡萄糖（FDG）正电子发射断层扫描（PET）生物标志物可用于检测啮齿动物肝肿瘤不可逆电穿孔（IRE）治疗反应的假说。
材料与方法 本机构动物护理和使用委员会批准了这项研究。大鼠在左中叶和左外侧叶接种 McA-RH7777 肝肿瘤细胞。允许肿瘤生长 7 天，以达到至少 5 毫米的最长直径，这通过磁共振（MR）成像得到证实。IRE 电极插入，在左中叶应用 8 个 100 微秒、2000 伏的脉冲以消融肿瘤组织。每个动物的左外侧叶肿瘤作为对照。在每个动物的 IRE 前后 3 天进行 PET/CT 和 MR 成像测量。IRE 后 14 天进行额外的 MR 成像测量。IRE 后 14 天的随访 MR 成像后，处死大鼠并采集肿瘤进行苏木精-伊红、CD34 和 caspase-3 染色。IRE 后 3 天计算最大标准化摄取值（ΔSUV）的变化。IRE 后 14 天通过轴向 T2 加权成像测量最大病变直径变化（ΔD）。比较 ΔSUV 和 ΔD。通过 caspase-3 染色的凋亡肿瘤细胞切片计算凋亡指数。计算 Pearson 相关系数以评估 IRE 治疗后 3 天的 ΔSUV 与 14 天的 ΔD（或凋亡指数）之间的关系。
结果 ΔSUV、ΔD 和凋亡指数在治疗和未治疗肿瘤之间差异有统计学意义（P <.001）。在治疗肿瘤中，IRE 后 3 天的 ΔSUV 与 IRE 后 14 天的 ΔD 之间存在很强的相关性（R = 0.66，P =.01），IRE 后 3 天的 ΔSUV 与 IRE 后 14 天的凋亡指数之间存在很强的相关性（R = 0.57，P =.04）。
结论 F-FDG PET 成像生物标志物可用于检测啮齿动物模型中 IRE 治疗肝肿瘤的治疗反应的早期变化。
放射学学会，2017 年。
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