Ortucu Serkan, Algur Omer Faruk
Department of Molecular Biology and Genetics, Erzurum Technical University, Erzurum, Turkey.
Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey.
J Pathog. 2017;2017:7628175. doi: 10.1155/2017/7628175. Epub 2017 Nov 8.
This study was conducted to assess highly pathogenic isolates to be used in biocontrol and to determine their potentials as mycopesticide. For this purpose, two isolates, which were locally isolated from , were chosen. Firstly, three suspensions were investigated at the degree of humidity of 65 ± 5% and 100% RH. Secondly, these strains were selected according to their tendency to mass production, tolerance to UV radiation, and capability of producing spore at the different temperatures. Finally, identification of the selected isolate was performed by using ITS rDNA analysis. Both tested fungal isolates were pathogenic to the . Mycelial growths of isolate AT076 at 20°C and 30°C were found to be greater than isolate AT007. It was observed that isolate AT076 had more spore production with 1.61 × 10 spore/disc at 30°C and 44.33% germination after UV radiation for 15 min. The numbers of spores per 5 mm disk area for isolates AT076 and AT007 were found to be 1.2 × 10 and 1.0 × 10. These results show that isolate AT076 was more virulent and more UV-tolerant and had higher tendency to mass production compared to isolate AT007 against . As a result of this study, isolate AT076 can be used in the biocontrol as mycopesticide.
本研究旨在评估用于生物防治的高致病性分离株,并确定其作为杀真菌剂的潜力。为此,选择了两个从当地分离得到的分离株。首先,在湿度为65±5%和相对湿度100%的条件下研究了三种悬浮液。其次,根据它们的大规模生产倾向、对紫外线辐射的耐受性以及在不同温度下产生孢子的能力来选择这些菌株。最后,通过ITS rDNA分析对所选分离株进行鉴定。两种测试的真菌分离株对……都具有致病性。发现分离株AT076在20°C和30°C下的菌丝生长大于分离株AT007。观察到分离株AT076在30°C下产生的孢子更多,每盘有1.61×10个孢子,紫外线辐射15分钟后发芽率为44.33%。分离株AT076和AT007每5毫米圆盘面积的孢子数分别为1.2×10和1.0×10。这些结果表明,与分离株AT007相比,分离株AT076对……更具毒性、更耐紫外线且具有更高的大规模生产倾向。作为本研究的结果,分离株AT076可作为杀真菌剂用于生物防治。