自动血液分析仪高荧光区域细胞群体与非典型淋巴细胞标记之间的相关性。
Correlation between the cell population in the automated hematology analyzer high-fluorescence region and atypical lymphocyte flags.
作者信息
Xie Hongjie, Wu Yue, Cui Wei
机构信息
Department of Clinical Laboratory, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
出版信息
J Clin Lab Anal. 2018 Jun;32(5):e22374. doi: 10.1002/jcla.22374. Epub 2017 Dec 21.
INTRODUCTION
During routine blood measurements using an automated hematology analyzer, two easily confused types of suspect flags related to lymphocytes often appear: atypical and immature lymphocytes. The aim of this study was to investigate the correlation of high fluorescence cell (HFC) parameter and lymphocyte flags determined from an automated hematology analyzer.
METHODS
A total of 93 patients affected by various pathologic conditions (viral infection, immunological disease, oncological disease and tumor) were divided into an "atypical lymphocytes" group ("atypical" for short), an "immature lymphocytes/blasts" flag group (abnormal), a mixed-flag group that includes "atypical lymphocytes" (mixed), and a non-flag group (non-flag).
RESULTS
The numbers of HFCs in the atypical, abnormal, mixed, and non-flag groups were 1.8% (0.9%-5.5%), 0.7% (0.1%-5.0%), 2.3% (1.2%-5.0%), and 0.8% (0.7%-1.2%), respectively. The HFCs of "atypical" appeared as a separate cluster with clear boundaries. The HFCs of "abnormal" as an unclear boundaries, and it was difficult to accurately distinguish between the HFCs from the immature lymphocytes and the normal lymphocytes. The lower limit of HFC when the atypical lymphocyte flag appeared was 0.04 × 10 /L. The number of HFCs was similar to atypical lymphocytes detected by microscopy and CD19 CD20 CD27 cells by flow cytometry at 78% and 76%, respectively. The number of HFCs detected in "atypical" and CD19 CD20 CD27 cells showed good consistency (r = .715), whereas the consistency was poorest for "abnormal" (r = .176).
CONCLUSION
It demonstrates that HFCs reflects atypical lymphocytes better than immature lymphocytes/blasts.
引言
在使用自动血液分析仪进行常规血液检测时，经常会出现两种容易混淆的与淋巴细胞相关的可疑标记：非典型淋巴细胞和未成熟淋巴细胞。本研究的目的是探讨自动血液分析仪测定的高荧光细胞（HFC）参数与淋巴细胞标记之间的相关性。
方法
将93例患有各种病理状况（病毒感染、免疫疾病、肿瘤疾病和肿瘤）的患者分为“非典型淋巴细胞”组（简称“非典型”）、“未成熟淋巴细胞/原始细胞”标记组（异常）、包含“非典型淋巴细胞”的混合标记组（混合）和无标记组（无标记）。
结果
非典型组、异常组、混合组和无标记组的HFC数量分别为1.8%（0.9%-5.5%）、0.7%（0.1%-5.0%）、2.3%（1.2%-5.0%）和0.8%（0.7%-1.2%）。“非典型”的HFC呈现为边界清晰的单独一簇。“异常”的HFC边界不清晰，难以准确区分未成熟淋巴细胞的HFC和正常淋巴细胞的HFC。出现非典型淋巴细胞标记时HFC的下限为0.04×10⁹/L。HFC数量与显微镜检测的非典型淋巴细胞以及流式细胞术检测的CD19⁺CD20⁺CD27⁺细胞数量分别相似，为78%和76%。“非典型”中检测到的HFC数量与CD19⁺CD20⁺CD27⁺细胞显示出良好的一致性（r = 0.715），而“异常”的一致性最差（r = 0.176）。
结论
结果表明，HFC反映非典型淋巴细胞的能力优于未成熟淋巴细胞/原始细胞。
Zotero 插件