基于芬顿反应的 Mn 掺杂 NaYF:Yb,Er 上转换纳米粒子用于尿酸检测。
Mn-doped NaYF:Yb,Er upconversion nanoparticles for detection of uric acid based on the Fenton reaction.
机构信息
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Anhui Key Laboratory of Chemo-Biosensing, Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
出版信息
Talanta. 2018 Apr 1;180:120-126. doi: 10.1016/j.talanta.2017.12.016. Epub 2017 Dec 15.
A novel fluorescence method for the determination of hydrogen peroxide (HO) and uric acid (UA) was developed. The procedure was based on the hydroxyl radicals (·OH), which effectively quenched the fluorescence of the Mn-doped NaYF:Yb,Er upconversion nanoparticles (UCNPs). Based on the property of Mn-doped NaYF:Yb,Er upconversion nanoparticles, the Fenton reaction and enzymatic reaction of uric acid, this method could be used for highly sensitive detection of HO and uric acid. Under optimal conditions, we observed that the fluorescence quenching signal showed good linearity with the HO concentration in the range of 3.00×10 M ~ 6.00×10 M, and the detection limit of this assay was 1.30×10 M. Meanwhile, the linear concentration range for UA was 4.00×10 M ~ 1.00×10 M, and the lower limit of detection was 1.90×10 M. Furthermore, the developed method was successfully used for the determination of UA levels in human serum with satisfactory results.
一种用于测定过氧化氢（HO）和尿酸（UA）的新型荧光法被开发出来。该方法基于羟基自由基（·OH），它能有效地猝灭 Mn 掺杂的 NaYF:Yb,Er 上转换纳米粒子（UCNPs）的荧光。基于 Mn 掺杂的 NaYF:Yb,Er 上转换纳米粒子的性质，以及尿酸的 Fenton 反应和酶促反应，该方法可用于高灵敏度检测 HO 和尿酸。在最佳条件下，我们观察到荧光猝灭信号与 HO 浓度在 3.00×10 M6.00×10 M 范围内呈现良好的线性关系，该测定的检测限为 1.30×10 M。同时，UA 的线性浓度范围为 4.00×10 M1.00×10 M，检测下限为 1.90×10 M。此外，该方法成功地用于人血清中 UA 水平的测定，结果令人满意。
Zotero 插件