生理学研究中抗体使用的作者和审稿人指南。
Guidelines for authors and reviewers on antibody use in physiology studies.
机构信息
Department of Physiology, Pharmacology and Medicine, Sarver Heart Center, College of Medicine, University of Arizona , Tucson, Arizona.
Mississippi Center for Heart Research, Department of Physiology and Biophysics, University of Mississippi Medical Center , Jackson, Mississippi.
出版信息
Am J Physiol Heart Circ Physiol. 2018 Apr 1;314(4):H724-H732. doi: 10.1152/ajpheart.00512.2017. Epub 2018 Jan 5.
Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/ .
抗体的使用是心血管生理学研究的一个关键组成部分，抗体被用于监测蛋白质丰度（免疫印迹分析）和蛋白质表达和定位（通过免疫组织化学在组织中，通过免疫细胞化学在细胞中）。随着关于如何提高重现性和严格性的讨论不断进行，本综述的目的是提供关于如何优化抗体使用的最佳实践指南，以提高免疫印迹分析和免疫组织化学方法的严谨性和重现性。请在 http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/ 上收听本文的相应播客。
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