Sysmex XN 9000检测贫血时新型红细胞和网织红细胞参数的稳定性

Stability of New Erythrocyte and Reticulocyte Parameters in Testing for Anemia on the Sysmex XN 9000.

作者信息

Schapkaitz Elise

机构信息

Department of Molecular Medicine and Hematology, University of Witwatersrand Medical School, Johannesburg, South Africa.

出版信息

Lab Med. 2018 Jul 5;49(3):219-225. doi: 10.1093/labmed/lmx095.

DOI:10.1093/labmed/lmx095

PMID:29365192

Abstract

BACKGROUND

New erythrocyte and reticulocyte parameters provide improved classification of anemia and monitoring of erythropoeitic activity. Parameters available on the Sysmex XN analyzers include the percentage of microcytic red blood cells (%Micro-R), percentage of macrocytic red blood cells (%Macro-R), percentage of hypochromic red blood cells (%Hypo-He), percentage of hyperchromic red blood cells (%Hyper-He), reticulocyte hemoglobin content (Ret-He), and immature reticulocyte fraction (IRF). However, use of these parameters is limited by specimen stability.

METHODS

To verify the stability of the new erythrocyte and reticulocyte parameters during prolonged storage, 20 blood specimens were analyzed on the Sysmex XN-9000 hematology analyzer. The specimens included healthy patients (60%) and patients with anemia (40%). The specimens were stored at room temperature (RT) and at 4° to 8°C. Analysis was performed after 12, 24, 48, and 72 hours of storage.

RESULTS

Reticulocyte parameters IRF and Ret-He were precise and stable for at least 72 hours after collection when stored at RT and 4° to 8°C. The volume-dependent parameters, %Macro-R and %Micro-R, were stable for less than 12 hours after collection at RT (mean [SD%], 6.55 [3.19%] and -20.70 [10.37%], respectively). Storage at 4° to 8°C showed a reduction in osmotic swelling. However, %Macro-R and %Micro-R were stable for less than 12 hours after collection (mean [SD%], 4.89 [2.02%] and -17.17 [8.38%], respectively). Similarly, %Hypo-He showed a mean (SD%) increase of 0.73 (4.05%) and %Hyper-He showed a decrease of -0.70 (9.72%) at less than 12 hours after storage at 4° to 8°C.

CONCLUSION

New reticulocyte parameters stored at RT and 4° to 8°C are suitable for testing on the Sysmex XN analyzer.

摘要

背景

新的红细胞和网织红细胞参数有助于改进贫血的分类及对红细胞生成活性的监测。Sysmex XN分析仪可提供的参数包括小红细胞百分比（%Micro-R）、大红细胞百分比（%Macro-R）、低色素红细胞百分比（%Hypo-He）、高色素红细胞百分比（%Hyper-He）、网织红细胞血红蛋白含量（Ret-He）以及未成熟网织红细胞比例（IRF）。然而，这些参数的应用受标本稳定性的限制。

方法

为验证新的红细胞和网织红细胞参数在长时间储存期间的稳定性，在Sysmex XN-9000血液分析仪上对20份血液标本进行了分析。标本包括健康患者（60%）和贫血患者（40%）。标本分别在室温（RT）和4°至8°C下储存。在储存12、24、48和72小时后进行分析。

结果

当在室温及4°至8°C下储存时，网织红细胞参数IRF和Ret-He在采集后至少72小时内精确且稳定。与体积相关的参数，%Macro-R和%Micro-R，在室温下采集后不到12小时就不稳定了（平均值[标准差%]分别为6.55[3.19%]和-20.70[10.37%]）。在4°至8°C下储存显示渗透肿胀有所减轻。然而，%Macro-R和%Micro-R在采集后不到12小时就不稳定了（平均值[标准差%]分别为4.89[2.02%]和-17.17[8.38%]）。同样，在4°至8°C下储存不到l2小时时，%Hypo-He平均（标准差%）升高了0.73（4.05%），%Hyper-He下降了-0.70（9.72%）。