Liu Dafei, Liu Fei, Guo Dongchun, Hu Xiaoliang, Li Zhijie, Li Zhigang, Ma Jianzhang, Liu Chunguo
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, China.
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China.
J Vet Med Sci. 2019 Jul 19;81(7):1040-1042. doi: 10.1292/jvms.17-0442. Epub 2018 Jan 23.
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10 TCID for CDV, 10 TCID for CPV and 10 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.
为了在实际中快速区分犬瘟热病毒（CDV）、犬细小病毒（CPV）和犬科布病毒（CaKoV），开发了一种一步多重PCR/RT-PCR检测方法，其对CDV的检测限为10个TCID，对CPV的检测限为10个TCID，对CaKoV的检测限为10个拷贝。该方法不会从其他犬病毒中扩增出非特异性DNA或RNA。因此，该检测方法为犬CDV、CPV和CaKoV的快速临床检测和流行病学监测提供了一种灵敏的工具。
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