Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, People's Republic of China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, People's Republic of China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, Jiangsu Province, People's Republic of China.
Int J Biol Macromol. 2018 May;111:1027-1031. doi: 10.1016/j.ijbiomac.2018.01.147. Epub 2018 Jan 31.
Small heat shock proteins (sHSPs) are conserved among insects and play an important role in the regulation of many biological processes, including temperature stress, abiotic stress, immune responses, metamorphosis, and embryo development. Antheraea pernyi is an economically valuable silk-producing moth and source of insect food containing high-quality protein. The aim of this study was to quantify expression of the ApsHSP21 gene in response to pathogen-associated molecular patterns (PAMPs) and nucleopolyhedrovirus (NPV) challenge. The deduced ApsHSP21 protein sequence consists of 186 residues with a calculated molecular mass of 21.0 kDa and an isoelectronic point (pI) of 6.63. The protein contains a conserved α-crystallin domain (ACD), and includes two casein kinase II phosphorylation sites, a protein kinase C phosphorylation site, two tyrosine kinase phosphorylation sites, and various polypeptide binding sites. Phylogenetic analysis revealed that ApsHSP21 is closely related to homologs from other insects. Real-time quantitative reverse transcription PCR (qRT-PCR) analysis revealed that expression of ApsHSP21 was significantly up-regulated at different timepoints following simulated pathogen challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), glucan, and NPV. The results suggest sHSP21 is involved in innate immune responses in A. pernyi.
小热休克蛋白（sHSPs）在昆虫中保守存在，在许多生物学过程的调节中发挥着重要作用，包括温度应激、非生物应激、免疫反应、变态和胚胎发育。柞蚕是一种具有经济价值的产丝蛾类昆虫，也是昆虫食品的来源，其富含高质量的蛋白质。本研究旨在定量检测 ApsHSP21 基因对病原体相关分子模式（PAMPs）和核型多角体病毒（NPV）挑战的应答表达。推测的 ApsHSP21 蛋白序列由 186 个残基组成，计算分子量为 21.0 kDa，等电点（pI）为 6.63。该蛋白包含一个保守的 α-晶体蛋白结构域（ACD），并包含两个酪蛋白激酶 II 磷酸化位点、一个蛋白激酶 C 磷酸化位点、两个酪氨酸激酶磷酸化位点以及各种多肽结合位点。系统发育分析表明，ApsHSP21 与其他昆虫的同源物密切相关。实时定量反转录 PCR（qRT-PCR）分析显示，在模拟病原体 LPS、PGN、葡聚糖和 NPV 挑战后，不同时间点 ApsHSP21 的表达均显著上调。这些结果表明 sHSP21 参与了柞蚕的先天免疫反应。
Zotero 插件