受磷蛋白在心肌调节中的假定作用。

The putative role of phospholamban in the regulation of the heart muscle.

作者信息

Holtzhauer M, Sydow H, Levchenko T S

出版信息

Biomed Biochim Acta. 1986;45(1-2):S233-6.

Abstract

We describe the 50fold purification of phospholamban, the production of antibodies against it and preliminary experiments of reconstitution of purified phospholamban into vesicles of skeletal sarcoplasmic reticulum (SR). Purified phospholamban migrates in a modified Laemmli SDS polyacrylamide gel electrophoresis (PAGE) system with a relative molecular mass (Mr) of 22 kD and 6 kD. The higher Mr form is detectable by immunoreaction on Western blots only in heart SR preparations, whereas the low Mr form is also present in sarcolemmal preparations. No immunoreactivity was found in SR of skeletal muscle. Reconstituted skeletal SR vesicles were not influenced by phospholamban in respect to their Ca++-ATPase activity and calcium accumulation rate, but the obtained data suggest that phospholamban alters the calcium storage capacity of SR.

摘要

我们描述了受磷蛋白的50倍纯化、针对它的抗体的产生以及将纯化的受磷蛋白重构到骨骼肌肌浆网（SR）囊泡中的初步实验。纯化的受磷蛋白在改良的Laemmli SDS聚丙烯酰胺凝胶电泳（PAGE）系统中迁移，相对分子质量（Mr）为22 kD和6 kD。较高Mr形式仅在心脏SR制剂的蛋白质免疫印迹反应中可检测到，而低Mr形式也存在于肌膜制剂中。在骨骼肌的SR中未发现免疫反应性。重构的骨骼肌SR囊泡在其Ca++ -ATP酶活性和钙积累速率方面不受受磷蛋白的影响，但获得的数据表明受磷蛋白改变了SR的钙储存能力。