使用Illumina BeadArray技术对人类胶质母细胞瘤进行全基因组DNA甲基化分析。
Whole Genome DNA Methylation Analysis of Human Glioblastoma Using Illumina BeadArrays.
作者信息
Serrano Jonathan, Snuderl Matija
机构信息
Department of Pathology, New York University School of Medicine, New York, NY, USA.
出版信息
Methods Mol Biol. 2018;1741:31-51. doi: 10.1007/978-1-4939-7659-1_2.
In this chapter, we describe the use of Illumina Infinium HD Assay in conjunction with Illumina's EPIC Methylation 8-sample array platform to obtain glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue and is compatible with an Illumina iScan or HiScan system.
在本章中，我们描述了如何将Illumina Infinium HD检测与Illumina的EPIC甲基化8样本阵列平台结合使用，以获得胶质母细胞瘤分子图谱。该过程持续四天，一名实验室技术人员即可完成。此方法起始DNA输入量低至250 ng，灵活性高，既可以从福尔马林固定石蜡包埋（FFPE）组织或新鲜组织提取的DNA开始，并且与Illumina iScan或HiScan系统兼容。
Zotero 插件