Individual and combined effect of and gene polymorphisms in lung cancer.

Lung cancer (LC) is the second common and with the highest mortality oncological disease. Specific biomarkers for its diagnostics, treatment, and prognosis are still under the investigations. Aim of our study was to evaluate the relationship between the polymorphisms of TP53 pathway genes , , , the polymorphisms of HPV-associated genes , , , and HPV infection with survival of LC patients. SNPs were genotyped using PCR-RFLP. qRT-PCR was used to detect, identify, and quantify HPV. No statistically significant differences were detected between individual SNPs and patient survival with stage I-IV LC. Cluster analysis of SNPs in genes A/A, wt/Δ32, C/T, T/T showed possible association with the worse survival. Patients who were diagnosed with C/T polymorphic variant of gene tend not to survive stage III-IV LC ( .12). There is a tendency between MDM2 gene T/T variant and worse survival of patients diagnosed with late stage LC ( .11). HPV infection is very rear among LC patients (3 of 92). Overall, there is a link, although statistically insignificant, between specific SNPs and LC patient survival frequency and time, meanwhile the combination of specific SNPs showed a statistically significant measure. In conclusion, we determined statistically significant ( .04) link between the poor survival of LC patients after surgery and the combination of polymorphic variants C/T of the and T/T of the genes, whereas individually these SNPs do not show significant relationship with the survival of patients after surgery.