[及其侧翼区域三个单核苷酸多态性（SNP）组成的SNP-STR在亲子鉴定中的应用]

[Application of SNP-STR Composed by and Three SNPs of Its Flanking Region in Paternity Testing].

作者信息

Gao J S, Ye Y, Hou Y P

机构信息

Institute of Forensic Science, Tibet Autonomous Region Public Security Department, Lhasa 850000, China.

West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

出版信息

Fa Yi Xue Za Zhi. 2017 Dec;33(6):607-610. doi: 10.3969/j.issn.1004-5619.2017.06.008. Epub 2017 Dec 25.

DOI:10.3969/j.issn.1004-5619.2017.06.008

PMID:29441768

Abstract

OBJECTIVES

To develop a SNP-STR haplotype by consisting of the SNP and STR genetic markers, both of which locate in a haplotype block. To investigate its distribution in Han population from Chengdu, and explore its application in some special cases of paternity testing.

METHODS

, one of the high mutation rate STR markers in combined DNA index system （CODIS）, and three SNP loci rs8089331, rs8094489 and rs7236090 in its flanking region, were chosen to establish SNP-STR. Its haplotype was obtained by nested allele-specific polymerase chain reaction, and the relevant distribution of 75 unrelated individuals were investigated in Han population from Chengdu. The SNP-STR haplotype was tentatively applied in duo paternity testing cases with incompatibility.

RESULTS

The SNP-STR typing method was established and a total of 43 haplotypes were obtained successfully in Han population from Chengdu. Its polymorphism was 0.948 6, and duo paternity testing cases were resolved by this method.

CONCLUSIONS

SNP-STR shows high diversity and can be applied in the identifications of some special paternity testing cases.

摘要

目的

由位于单倍型块中的单核苷酸多态性（SNP）和短串联重复序列（STR）遗传标记构建SNP-STR单倍型。研究其在成都汉族人群中的分布，并探讨其在一些特殊亲子鉴定案例中的应用。

方法

选择联合DNA索引系统（CODIS）中高突变率的STR标记之一及其侧翼区域的3个SNP位点rs8089331、rs8094489和rs7236090构建SNP-STR。通过巢式等位基因特异性聚合酶链反应获得其单倍型，并调查成都汉族人群中75名无关个体的相关分布情况。将该SNP-STR单倍型初步应用于存在不相容情况的二联体亲子鉴定案例。