食管鳞状细胞癌患者肿瘤组织中miR-34a的表达水平
Expression Level of miR-34a in Tumor Tissue from Patients with Esophageal Squamous Cell Carcinoma.
作者信息
Asadi Milad, Shanehbandi Dariush, Mohammadpour Hanieh, Hashemzadeh Shahriyar, Sepehri Bita
机构信息
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
出版信息
J Gastrointest Cancer. 2019 Jun;50(2):304-307. doi: 10.1007/s12029-018-0060-0.
BACKGROUND
miR-34a has been shown to be involved in P53 regulation. In this study, we aimed to evaluate the expression level of miR-34a in esophageal cancer and compare it with that of the normal marginal tissues.
METHODS
Tumor and marginal tissues were obtained from 50 patients with esophageal cancer. After RNA extraction, expression level of miR-34a was determined using SYBR green master mix and real-time quantitative PCR.
RESULTS
It was observed that there was a downregulation of miR-34a in tumoral tissue of esophageal patients in comparison to normal marginal tissues. Moreover, the expression level of miR-34a was correlated with clinicopathological specifications of the patients.
CONCLUSIONS
miR-34a may be involved in the pathogenesis and development of esophageal cancer and have the potential to be used as a diagnostic and therapeutic marker in esophageal cancer.
背景
已证明miR-34a参与p53调控。在本研究中，我们旨在评估食管癌中miR-34a的表达水平，并将其与正常边缘组织的表达水平进行比较。
方法
从50例食管癌患者中获取肿瘤组织和边缘组织。提取RNA后，使用SYBR Green Master Mix和实时定量PCR测定miR-34a的表达水平。
结果
观察到与正常边缘组织相比，食管癌患者肿瘤组织中miR-34a表达下调。此外，miR-34a的表达水平与患者的临床病理特征相关。
结论
miR-34a可能参与食管癌的发病机制和发展，并有潜力用作食管癌的诊断和治疗标志物。
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