McAllen Robin M, Shafton Anthony D, Bratton Bradford O, Trevaks David, Furness John B
Florey Institute of Neuroscience & Mental Health Parkville, Victoria 3010, Australia.
Department of Anatomy & Neuroscience, University of Melbourne, Parkville, Victoria 3010, Australia.
Bioelectron Med (Lond). 2018 Jan;1(1):21-27. doi: 10.2217/bem-2017-0001. Epub 2017 Nov 3.
Vagal nerve stimulation is widely used therapeutically but the fiber groups activated are often unknown.
To establish a simple protocol to define stimulus thresholds for vagal A, B and C fibers.
The intact left or right cervical vagus was stimulated with 0.1 ms pulses in spontaneously breathing anesthetized rats. Heart and respiratory rate responses to vagal stimulation were recorded. The vagus was subsequently cut distally, and mass action potentials to the same stimuli were recorded.
Stimulating at either 50 Hz for 2 s or 2 Hz for 10 s at experimentally determined strengths revealed A, B and C fiber thresholds that were related to respiratory and heart rate changes.
Our simple protocol discriminates vagal A, B and C fiber thresholds .
迷走神经刺激在治疗中被广泛应用,但所激活的纤维组往往并不明确。
建立一种简单的方案来确定迷走神经A、B和C纤维的刺激阈值。
在自主呼吸的麻醉大鼠中,用0.1毫秒的脉冲刺激完整的左侧或右侧颈迷走神经。记录迷走神经刺激引起的心率和呼吸频率反应。随后在远端切断迷走神经,并记录对相同刺激的复合动作电位。
以实验确定的强度在50赫兹刺激2秒或在2赫兹刺激10秒时,可揭示与呼吸和心率变化相关的A、B和C纤维阈值。
我们的简单方案能够区分迷走神经A、B和C纤维阈值。