Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Sci Rep. 2018 Feb 28;8(1):3786. doi: 10.1038/s41598-018-22098-y.
The present study aim to design a liposomal electrochemical sensor using 1, 2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and dioleoylphosphatidylethanolamine(DOPE), chimeric probes and p19, it has been considered as a caliper molecule as well. Also the competitor structural hybrid (RNA) was used to detect three types of miRs in one screen printed electrode modified by gold nanoparticle (SCPE/GNP). In this purpose, the sensor signal stabilized when the cationic DOTAP-DOPE with hybrids of the chimeric probes (Stem, M-linear) sandwiched in order to detect 221-124a miRs. Given the lack of accessibility to RNA-miRs segments of chimeric probes, p19 inhibited the electrochemical reaction and shifted signal to off. After that p19 connected with the free hybrid of T-linear/21miR (just RNA) as competing for structure and the signal was shifted to ON, again. In this study, the electrochemical measurements were performed between the potentials at -0.4 V and +0.4 V with 1 mM [Fe(CN)6]-3-/4 which DOTAP-DOPE acted as an enhancer layer in the electrostatically reaction. This sensor determines as low as 0.4 fM of miRNA with high selectivity and specificity for sequential analysis of 124a-221-21 miRs in just 2 h.
本研究旨在设计一种使用 1,2-二油酰基-3-三甲铵丙烷 (DOTAP) 和二油酰基磷脂酰乙醇胺 (DOPE)、嵌合探针和 p19 的脂质体电化学传感器，p19 也被认为是一种卡尺分子。此外，还使用竞争结构杂合体 (RNA) 在金纳米粒子修饰的丝网印刷电极 (SCPE/GNP) 上检测三种类型的 miR。为此，当带有嵌合探针 (Stem、M-线性) 混合物的阳离子 DOTAP-DOPE 稳定传感器信号时，以检测 221-124a miRs。由于无法接近嵌合探针的 RNA-miRs 片段，p19 抑制了电化学反应并将信号切换为关闭。之后，p19 与游离的 T-线性/21miR 杂交体 (仅 RNA) 连接，作为结构竞争物，信号再次切换为开启。在这项研究中，在 -0.4 V 和 +0.4 V 之间的电位下进行电化学测量，其中 DOTAP-DOPE 作为静电反应中的增强层。该传感器的检测下限低至 0.4 fM，对 124a-221-21 miR 的顺序分析具有高选择性和特异性，仅需 2 小时。
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