整合素连接激酶在结直肠癌中的表达与上皮-间质转化、癌症干细胞标志物及化疗耐药相关。
ILK Expression in Colorectal Cancer Is Associated with EMT, Cancer Stem Cell Markers and Chemoresistance.
作者信息
Tsoumas Dimitrios, Nikou Sofia, Giannopoulou Efstathia, Champeris Tsaniras Spyridon, Sirinian Chaido, Maroulis Ioannis, Taraviras Stavros, Zolota Vassiliki, Kalofonos Haralabos P, Bravou Vasiliki
机构信息
Department of Anatomy-Histology-Embryology, Medical School, University of Patras, Patras, Greece.
Clinical Oncology Laboratory, University of Patras Medical School, Patras, Greece.
出版信息
Cancer Genomics Proteomics. 2018 Mar-Apr;15(2):127-141. doi: 10.21873/cgp.20071.
BACKGROUND/AIM: Epithelial-mesenchymal transition (EMT) and cancer stem cells (CSC) are critically implicated in cancer metastasis and chemoresistance. Herein, we investigated integrin-linked kinase (ILK)'s role in human colon cancer (CRC) progression and chemoresistance in relation to EMT and CSC markers.
PATIENTS AND METHODS
Expression of ILK, EMT and CSC markers were evaluated by immunohistochemistry in 149 CRC samples. We also generated colon cancer cells resistant to 5-FU and oxaliplatin and studied the effect of ILK inhibition on drug response by MTT assay and on EMT and CSC markers' expression.
RESULTS
ILK expression in human CRC correlates with EMT and CSC markers and is associated with metastasis and chemoresistance. ILK inhibition increases sensitivity of resistant cells to 5-FU and oxaliplatin and reduces the levels of EMT and CSC markers in 5-FU resistant cells.
CONCLUSION
ILK overexpression in human CRC associates with EMT and CSC traits, contributing to tumor progression and chemoresistance.
背景/目的：上皮-间质转化（EMT）和癌症干细胞（CSC）与癌症转移和化疗耐药密切相关。在此，我们研究了整合素连接激酶（ILK）在人类结肠癌（CRC）进展和化疗耐药中与EMT和CSC标志物相关的作用。
患者和方法
通过免疫组织化学评估149例CRC样本中ILK、EMT和CSC标志物的表达。我们还生成了对5-氟尿嘧啶和奥沙利铂耐药的结肠癌细胞，并通过MTT法研究了ILK抑制对药物反应的影响以及对EMT和CSC标志物表达的影响。
结果
人类CRC中ILK的表达与EMT和CSC标志物相关，并与转移和化疗耐药相关。ILK抑制增加了耐药细胞对5-氟尿嘧啶和奥沙利铂的敏感性，并降低了5-氟尿嘧啶耐药细胞中EMT和CSC标志物的水平。
结论
人类CRC中ILK的过表达与EMT和CSC特征相关，促进肿瘤进展和化疗耐药。
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