大鼠细胞与HT 1080人纤维肉瘤细胞的体细胞杂种中大鼠染色体的分离。

Segregation of rat chromosomes in somatic cell hybrids between rat cells and HT 1080 human fibrosarcoma cells.

作者信息

D'Ancona G G, Croce C M

出版信息

Hum Genet. 1979;53(1):17-21. doi: 10.1007/BF00289444.

DOI:10.1007/BF00289444

Abstract

We produced somatic cell hybrids between HT 1080-6TG human fibrosarcoma cells and either rat white blood cells (WBC) or cells directly derived from rat spleen. Karyologic and isozyme analyses of hybrid cells indicated that they preferentially lose rat chromosomes. Hypoxanthine-aminopterine thymidine-selected hybrid clones expressing rat hypoxanthine phosphoribosyltransferase (HPRT), glucose-6-phosphate dehydrogenase (G6PD), and phosphoglycerate kinase (PGK) and containing the rat X chromosome were counterselected in a medium containing 30 micrograms/ml of 6-thioguanine. Concordant loss of the rat X chromosome and of the expression of rat HPRT and G6PD was observed in the hybrid clones.

摘要

我们制备了HT 1080 - 6TG人纤维肉瘤细胞与大鼠白细胞（WBC）或直接来源于大鼠脾脏的细胞之间的体细胞杂种。杂种细胞的染色体组学和同工酶分析表明，它们优先丢失大鼠染色体。在含有30微克/毫升6 - 硫鸟嘌呤的培养基中对表达大鼠次黄嘌呤磷酸核糖转移酶（HPRT）、葡萄糖-6-磷酸脱氢酶（G6PD）和磷酸甘油酸激酶（PGK）并含有大鼠X染色体的次黄嘌呤-氨基蝶呤-胸腺嘧啶核苷选择的杂种克隆进行反选择。在杂种克隆中观察到大鼠X染色体以及大鼠HPRT和G6PD表达的一致性丢失。

相似文献

1

Segregation of rat chromosomes in somatic cell hybrids between rat cells and HT 1080 human fibrosarcoma cells.大鼠细胞与HT 1080人纤维肉瘤细胞的体细胞杂种中大鼠染色体的分离。

Hum Genet. 1979;53(1):17-21. doi: 10.1007/BF00289444.

2

The bovine genes for phosphoglycerate kinase, glucose-6-phosphate dehydrogenase, alpha-galactosidase, and hypoxanthine phosphoribosyltransferase are linked to the X chromosome in cattle-mouse cell hybrids.磷酸甘油酸激酶、葡萄糖-6-磷酸脱氢酶、α-半乳糖苷酶和次黄嘌呤磷酸核糖基转移酶的牛基因在牛-鼠细胞杂种中与X染色体相连。

Cytogenet Cell Genet. 1981;29(1):26-31. doi: 10.1159/000131548.

3

[Bovine chromosome mapping with the cell hybridization technic. Localization on the x chromosome of glucose-6-phosphate dehydrogenase, phosphoglycerate kinase, alpha-galactosidase A and hypoxanthine phosphoribosyltransferase].[利用细胞杂交技术进行牛染色体图谱分析。葡萄糖-6-磷酸脱氢酶、磷酸甘油酸激酶、α-半乳糖苷酶A和次黄嘌呤磷酸核糖基转移酶在X染色体上的定位]

Ann Genet. 1978 Dec;21(4):197-202.

4

Regional localization of the genes for human HEXB. PGK, GALA. HPRT, G6PD by somatic cell hybridization.通过体细胞杂交对人类己糖胺酶B、磷酸甘油酸激酶、半乳糖激酶、次黄嘌呤-鸟嘌呤磷酸核糖转移酶、葡萄糖-6-磷酸脱氢酶基因进行区域定位。

Ann Genet. 1981;24(2):89-92.

5

Mapping a marsupial X chromosome using kangaroo-mouse somatic cell hybrids.利用袋鼠-小鼠体细胞杂种绘制有袋类动物X染色体图谱。

Cytogenet Cell Genet. 1981;29(3):127-37. doi: 10.1159/000131561.

6

Human X-Linked genes regionally mapped utilizing X-autosome translocations and somatic cell hybrids.利用X-常染色体易位和体细胞杂种对人类X连锁基因进行区域定位。

Proc Natl Acad Sci U S A. 1975 Jun;72(6):2125-9. doi: 10.1073/pnas.72.6.2125.

7

[Statistical methods of radiation hybrid mapping: analysis of a small number of genes].

Genetika. 1999 Mar;35(2):392-9.

8

Subchromosomal localization and order of GLA, PGK1, HPRT, and G6PD loci on the X chromosome of the American mink (Mustela vison).美洲水貂（鼬属水貂）X染色体上GLA、PGK1、HPRT和G6PD基因座的亚染色体定位及顺序

Cytogenet Cell Genet. 1988;48(1):2-5. doi: 10.1159/000132574.

9

[Gene mapping in the pig (Sus scrofa 1.). I. Study of two syntenic groups G6PD, PGK, HPRT and PKM2, MPI (author's transl)].[猪（野猪1.）的基因定位。I. 两个同线基因群G6PD、PGK、HPRT和PKM2、MPI的研究（作者译）]

Ann Genet. 1980;23(1):15-21.

10

The gene map of the rabbit (Oryctolagus cuniculus L.) I. Synteny between the rabbit gene loci coding for HPRT, PGK, G6PD, and GLA: their localization on the X chromosome.兔（穴兔）的基因图谱。I.编码次黄嘌呤磷酸核糖转移酶、磷酸甘油酸激酶、葡萄糖-6-磷酸脱氢酶和α-半乳糖苷酶的兔基因位点间的同线性：它们在X染色体上的定位

Cytogenet Cell Genet. 1981;29(3):176-83. doi: 10.1159/000131566.

本文引用的文献

1

SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.体外成纤维细胞交配产生的杂种及其假定重组体的选择

Science. 1964 Aug 14;145(3633):709-10. doi: 10.1126/science.145.3633.709.

2

Loss of human genetic markers in man--Chinese hamster somatic cell hybrids.人类遗传标记在人-中国仓鼠体细胞杂种中的丢失

Nat New Biol. 1971 Nov 3;234(44):20-4. doi: 10.1038/newbio234020a0.

3

Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes.含有部分人类染色体互补物和功能性人类基因的人-鼠杂交细胞系。

Proc Natl Acad Sci U S A. 1967 Sep;58(3):1104-11. doi: 10.1073/pnas.58.3.1104.

4

Assignment of three gene loci (PGK, HGPRT, G6PD) to the long arm of the human X chromosome by somatic cell genetics.通过体细胞遗传学将三个基因位点（磷酸甘油酸激酶、次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶、葡萄糖 -6- 磷酸脱氢酶）定位于人类 X 染色体长臂。

Genetics. 1973 Aug;74(4):661-78. doi: 10.1093/genetics/74.4.661.

5

Polymorphism and linkage for mannosephosphate isomerase in Mus musculus.小家鼠中磷酸甘露糖异构酶的多态性与连锁关系

Biochem Genet. 1973 Jan;8(1):47-53. doi: 10.1007/BF00485556.

6

Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man.人类中苹果酸氧化还原酶脱羧基因定位于6号染色体，肽酶B和乳酸脱氢酶B基因定位于12号染色体。

Am J Hum Genet. 1973 Mar;25(2):200-7.

7

A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels.小鼠及体细胞杂种在淀粉凝胶中的酶多态性、连锁关系及电泳条件综述。

J Histochem Cytochem. 1973 Dec;21(12):1066-81. doi: 10.1177/21.12.1066.

8

A rapid fluorescence technique for electrophoretic identification of hypoxanthine phosphoribosyltransferase allozymes.一种用于电泳鉴定次黄嘌呤磷酸核糖基转移酶同工酶的快速荧光技术。

Biochem Genet. 1974 Nov;12(5):375-84. doi: 10.1007/BF00486642.

9

Isozymes and genetic control of NADP-malate dehydrogenase in mice.小鼠中NADP-苹果酸脱氢酶的同工酶与遗传控制

Arch Biochem Biophys. 1966 Oct;117(1):28-33. doi: 10.1016/0003-9861(66)90121-4.

10

Unidirectional loss of human chromosomes in rat-human hybrids.大鼠 - 人类杂交细胞系中人类染色体的单向丢失

Exp Cell Res. 1973 Jun;79(2):461-3. doi: 10.1016/0014-4827(73)90470-9.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。