Department of Applied Chemistry , Anhui Agricultural University , Hefei 230036 , China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry , The Chinese Academy of Sciences (CAS) , Beijing 100190 , China.
Anal Chem. 2018 Apr 17;90(8):4968-4971. doi: 10.1021/acs.analchem.7b05442. Epub 2018 Apr 2.
Electrochemical aptasensors generally include three elements, that is, recognition element, signal-transformation element, and regeneration element. In this study, a new adenosine triphosphate (ATP) aptasensor is developed by combining three elements into one DNA oligonucleotide chain. In the DNA oligonucleotide chain, DNA aptamer is used as the recognition element, ferrocene group attached at the 3'-end of the aptamer is used as the signal-transformation element, and azobenzene moiety embedded into the DNA chain is used as the regeneration element. In addition to the similar analytical properties with the traditional ones, the aptasensor developed here is easily regenerated with UV-light irradiation. The current response recorded on the aptasensor increases with increasing the concentration of ATP in the incubation solution and is linear with the logarithm of ATP concentration in the range from 1 nM to 100 μM. The limit of detection is 0.5 nM (S/N = 3). The basal level of ATP in the rat brain cortex microdialysate is determined to be 21.33 ± 4.1 nM ( n = 3). After being challenged with ATP, the aptasensor could be readily regenerated by UV-light irradiation for more than seven cycles. The regeneration of the aptasensor is proposed to be regulated by conversing azobenzene from its trans to cis form under UV irradiation.
电化学适体传感器通常包括三个元件，即识别元件、信号转换元件和再生元件。在本研究中，通过将三个元件组合成一条 DNA 寡核苷酸链，开发了一种新型的三磷酸腺苷（ATP）适体传感器。在 DNA 寡核苷酸链中，DNA 适体用作识别元件，连接在适体 3'端的二茂铁基团用作信号转换元件，嵌入 DNA 链中的偶氮苯部分用作再生元件。除了具有与传统传感器类似的分析性能外，这里开发的适体传感器还可以通过紫外光照射轻松再生。在适体传感器上记录的电流响应随孵育溶液中 ATP 浓度的增加而增加，并且在 1 nM 至 100 μM 的范围内与 ATP 浓度的对数呈线性关系。检测限为 0.5 nM（S/N = 3）。测定大鼠大脑皮层微透析液中 ATP 的基础水平为 21.33 ± 4.1 nM（n = 3）。用 ATP 进行挑战后，适体传感器可以通过紫外光照射轻松再生超过 7 个循环。提出适体传感器的再生是通过在紫外光照射下将偶氮苯从反式转化为顺式来调节的。
